Study on Biogenic Spindle-Shaped Iron-Oxide Nanoparticles by Pseudostaurosira trainorii in Field of Laser Desorption/Ionization Applications

Nanostructures-assisted laser desorption/ionization mass spectrometry (NALDI-MS) is gaining attention for the analysis of a wide range of molecules. In this present investigation, Pseudostaurosira trainorii mediated biosynthesized iron-oxide nanoparticles (IONPs) have been utilized as nanostructures...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2022-10, Vol.23 (19), p.11713
Main Authors: Roychoudhury, Piya, Golubeva, Aleksandra, Dąbek, Przemysław, Pryshchepa, Oleksandra, Sagandykova, Gulyaim, Pomastowski, Paweł, Gloc, Michał, Dobrucka, Renata, Kurzydłowski, Krzysztof, Buszewski, Bogusław, Witkowski, Andrzej
Format: Article
Language:English
Subjects:
Alanine
Algae
Aqueous solutions
Biocompatibility
biogenesis
Biomass
Carotenoids
Catalysis
Cell division
Decoloring
Desorption
diatom
Fever
Gold
Hyperthermia
Ionization
Iron
iron-oxide nanoparticles
Lasers
LDI-MS
Mass spectrometry
Mass spectroscopy
Molecular weight
nano-spindle
Nanomaterials
Nanoparticles
Nitrophenol
Pseudostaurosira trainorii
Scanning electron microscopy
Spectroscopy
Spectrum analysis
Spindles
Triglycerides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanostructures-assisted laser desorption/ionization mass spectrometry (NALDI-MS) is gaining attention for the analysis of a wide range of molecules. In this present investigation, Pseudostaurosira trainorii mediated biosynthesized iron-oxide nanoparticles (IONPs) have been utilized as nanostructures assisting ionization and desorption for laser desorption/ionization mass spectrometry (LDI-MS). The chain forming diatom, P. trainorii showed efficiency in the production of IONPs against 0.01 M Fe+3 (pH 2) aqueous solution at the intracellular and extracellular level. The whole biomass and external media turned dark orange in color after 3 days of reaction with Fe3+ solution. Scanning electron microscopic (SEM) images illustrated that the surface of Fe3+ exposed frustules of P. trainorii were entirely covered by synthesized nanostructures contrasting with the natural surface ornamentation of control cells. The IONPs loaded frustules also exhibited catalytic properties by decolorizing yellow colored nitrophenol after 3 h of reaction. Transmission electron microscopic (TEM) images confirmed that the produced particles are spindle-shaped with ~50–70 nm length and ~10–30 nm width. The biogenic IONPs were utilized as an inorganic matrix in LDI-MS and showed high sensitivity towards small molecules as glucose, alanine and triacylglycerols at nano- and picomolar level per spot, respectively. The presented biocompatible technique offers new perspectives in nanobiotechnology for the production of spindle-shaped IONPs that can be applied in future for the preparation of NALDI plates.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms231911713