Study on Biogenic Spindle-Shaped Iron-Oxide Nanoparticles by Pseudostaurosira trainorii in Field of Laser Desorption/Ionization Applications

• Published in: International journal of molecular sciences 2022-10, Vol.23 (19), p.11713
• Main Authors: Roychoudhury, Piya, Golubeva, Aleksandra, Dąbek, Przemysław, Pryshchepa, Oleksandra, Sagandykova, Gulyaim, Pomastowski, Paweł, Gloc, Michał, Dobrucka, Renata, Kurzydłowski, Krzysztof, Buszewski, Bogusław, Witkowski, Andrzej
• Format: Article
• Language: English
• Subjects: Alanine; Algae; Aqueous solutions; Biocompatibility; biogenesis; Biomass; Carotenoids; Catalysis; Cell division; Decoloring; Desorption; diatom; Fever; Gold; Hyperthermia; Ionization; Iron; iron-oxide nanoparticles; Lasers; LDI-MS; Mass spectrometry; Mass spectroscopy; Molecular weight; nano-spindle; Nanomaterials; Nanoparticles; Nitrophenol; Pseudostaurosira trainorii; Scanning electron microscopy; Spectroscopy; Spectrum analysis; Spindles; Triglycerides
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231911713

Nanostructures-assisted laser desorption/ionization mass spectrometry (NALDI-MS) is gaining attention for the analysis of a wide range of molecules. In this present investigation, Pseudostaurosira trainorii mediated biosynthesized iron-oxide nanoparticles (IONPs) have been utilized as nanostructures assisting ionization and desorption for laser desorption/ionization mass spectrometry (LDI-MS). The chain forming diatom, P. trainorii showed efficiency in the production of IONPs against 0.01 M Fe+3 (pH 2) aqueous solution at the intracellular and extracellular level. The whole biomass and external media turned dark orange in color after 3 days of reaction with Fe3+ solution. Scanning electron microscopic (SEM) images illustrated that the surface of Fe3+ exposed frustules of P. trainorii were entirely covered by synthesized nanostructures contrasting with the natural surface ornamentation of control cells. The IONPs loaded frustules also exhibited catalytic properties by decolorizing yellow colored nitrophenol after 3 h of reaction. Transmission electron microscopic (TEM) images confirmed that the produced particles are spindle-shaped with ~50–70 nm length and ~10–30 nm width. The biogenic IONPs were utilized as an inorganic matrix in LDI-MS and showed high sensitivity towards small molecules as glucose, alanine and triacylglycerols at nano- and picomolar level per spot, respectively. The presented biocompatible technique offers new perspectives in nanobiotechnology for the production of spindle-shaped IONPs that can be applied in future for the preparation of NALDI plates.