Trichoderma: Population Structure and Genetic Diversity of Species with High Potential for Biocontrol and Biofertilizer Applications

• Published in: Applied Microbiology (Basel) 2024-05, Vol.4 (2), p.875-893
• Main Authors: Ismaiel, Adnan, Lakshman, Dilip K., Jambhulkar, Prashant P., Roberts, Daniel P.
• Format: Article
• Language: English
• Subjects: biocontrol agent; biofertilizer; biofertilizers; biological control; commercialization; endophytes; genetic variation; microbiology; plant tissues; population structure; population structure and genetic diversity; soil; species; species diversity; surveys; sustainable agriculture; Trichoderma; Trichoderma species
• ISSN: 2673-8007; 2673-8007
• DOI: 10.3390/applmicrobiol4020060

Certain Trichoderma isolates provide biofertilizer, biocontrol, and other plant-beneficial activities while inhabiting the soil or internal plant tissue, and their use in agricultural systems can contribute to sustainable food production. It is thought that colonization of soil or internal plant tissue is fundamental for biocontrol and biofertilizer applications. Our collective analyses of prior surveys, where the tef1α sequence was almost exclusively used to identify Trichoderma species, showed that isolates from the Harzianum complex clade, the T. asperellum/T. asperelloides group, T. virens, T. hamatum, and T. atroviride were prevalent in soil and/or as endophytes. Population structure and genetic diversity based on the genetic markers tef1α, rpb2, and ITS were investigated, and new lineages with statistical bootstrap support within T. atroviride, T. asperellum, T. hamatum, and T. virens populations were found. The nearest relatives of some of these species were also revealed. Choosing isolates from among more than 500 known Trichoderma species for use in non-targeted evaluation screens for biocontrol or biofertilizer applications is time-consuming and expensive. Preferentially selecting isolates from T. atroviride, T. asperellum/T. asperelloides, T. hamatum, the T. harzianum complex clade, T. virens, and possibly nearest relatives may speed the identification of candidates for commercialization due to the demonstrated ability of these species to successfully inhabit the soil and internal plant tissue. To our knowledge, this is the first report where dominant soil and endophytic Trichoderma species were identified from past survey data and population structure and genetic diversity analyses conducted.