Ischemic Stroke and Dietary Vitamin B12 Deficiency in Old-Aged Females: Impaired Motor Function, Increased Ischemic Damage Size, and Changed Metabolite Profiles in Brain and Cecum Tissue

A vitamin B12 deficiency (vit. B12 def.) is common in the elderly, because of changes in metabolism. Clinical studies have reported that a vit. B12 def. results in worse outcome after stroke, and the mechanisms through which a vit. B12 def. changes the brain requires further investigation. This stud...

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Bibliographic Details
Published in:Nutrients 2022-07, Vol.14 (14), p.2960
Main Authors: Poole, Joshua, Jasbi, Paniz, Pascual, Agnes S, North, Sean, Kwatra, Neha, Weissig, Volkmar, Gu, Haiwei, Bottiglieri, Teodoro, Jadavji, Nafisa M
Format: Article
Language:English
Subjects:
aging
Animal tissues
Animals
Apoptosis
Behavior
Brain
Brain damage
Brain injury
Cecum
Choline
Cyanocobalamin
Diet
Female
Females
Folic Acid
Homocysteine
Ischemia
Ischemic Stroke
Liver
Males
Metabolism
Metabolites
Metabolomics
Mice
Mitochondria
motor function
Nutrient deficiency
Nutrition research
Risk factors
Somatosensory cortex
Stroke
Tissue analysis
Tricarboxylic acid cycle
Vitamin B 12
Vitamin B 12 Deficiency
Vitamin B12
Women
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Summary:A vitamin B12 deficiency (vit. B12 def.) is common in the elderly, because of changes in metabolism. Clinical studies have reported that a vit. B12 def. results in worse outcome after stroke, and the mechanisms through which a vit. B12 def. changes the brain requires further investigation. This study investigated the role of vit. B12 def. on stroke outcome and mechanisms using aged female mice. Eighteen-month-old females were put on a control or vit. B12 def. diet for 4 weeks, after which an ischemic stroke was induced in the sensorimotor cortex. After damage, motor function was measured, the animals were euthanized, and tissues were collected for analysis. Vit. B12 def. animals had increased levels of total homocysteine in plasma and liver, and choline levels were also increased in the liver. Vit. B12 def. animals had larger damage volume in brain tissue and more apoptosis. The cecum tissue pathway analysis showed dysfunction in B12 transport. The analysis of mitochondrial metabolomics in brain tissue showed reduced levels of metabolites involved in the TCA cycle in vit. B12 def. animals. Motor function after stroke was impaired in vit. B12 def. animals. A dietary vit. B12 def. impairs motor function through increased apoptosis and changes in mitochondrial metabolism in brain tissue.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu14142960