GSTP1 Ile105Val Polymorphism in Serbian Patients with Pancreatic Diseases

• Published in: Journal of medical biochemistry 2011-01, Vol.30 (2), p.121-125
• Main Authors: Nikolic, Aleksandra, Stankovic, Marija, Nisevic, Ivan, Lukic, Snezana, Andelic-Jelic, Marina, Popovic, Dragan, Radojkovic, Dragica
• Format: Article
• Language: English
• Subjects: chronic pancreatitis; dijabetes melitus tipa 2; glutathione S-transferase P1 (GSTP1); glutation S-transferaza P1 (GSTP1); hronični pankreatitis; Ile105Val polymorphism; karcinom pankreasa; pancreatic cancer; polimorfizam Ile105Val; type 2 diabetes mellitus
• ISSN: 1452-8258; 1452-8266
• DOI: 10.2478/v10011-011-0001-y

The aim of the current preliminary case-control study was to identify glutathione S-transferase P1 (GSTP1) Ile105Val allele and genotype frequency and to evaluate its impact on susceptibility to pancreatic diseases in a Serbian population. This study has encompassed 157 patients with three major types of chronic pancreatic pathology: 47 with pancreatic cancer, 50 with chronic pancreatitis and 60 with type 2 diabetes mellitus, as well as 107 healthy individuals. The presence of GSTP1 Ile105Val polymorphism was analyzed using a PCR-RFLP method. Allele 105Val was less frequent in patients with pancreatic cancer (24.5%) and chronic pancreatitis (24.0%) and slightly more frequent in patients with type 2 diabetes mellitus (31.7%) in comparison to healthy individuals (29.9%), but the differences were not statistically significant. Distribution of Ile105Val polymorphism genotypes differed between the analyzed groups, but differences were also not statistically significant. There are only a few studies regarding the role of GSTP1 Ile105Val polymorphism in pancreatic diseases and their results are inconsistent. The significance of GSTP1 Ile105Val polymorphism for pancreatic pathology remains unclear and further studies are needed in order to elucidate its role in pancreatic diseases. Cilj ove studije bio je da se odredi učestalost polimorfizma Ile105Val u genu za glutation S-transferazu P1 (GSTP1) i proceni njegov uticaj na rizik za obolevanje od pankreasnih bolesti u srpskoj populaciji. Studija je obuhvatila 157 pacijenata sa najčešćim bolestima pankreasa: 47 sa karcinomom pankreasa, 50 sa hroničnim pankreatitisom i 60 sa dijabetes melitusom tipa 2, kao i 107 zdravih ispitanika. Prisustvo polimorfizma Ile105Val u genu za GSTP1 je analizirano metodom PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism). Učestalost alela 105Val je bila niža kod pacijenata sa karcinomom pankreasa (24,5%) i hroničnim pankreatitisom (24,0%) i neznatno povišena kod pacijenata sa dijabetes melitusom tipa 2 (31,7%) u odnosu na učestalost kod zdravih ispitanika (29,9%), ali razlike nisu bile statistički značajne. Učestalost pojedinih genotipova polimorfizma Ile105Val se razlikovala među analiziranim grupama, ali razlike takođe nisu bile statistički značajne. Samo nekoliko studija se do sada bavilo ulogom GSTP1 Ile105Val polimorfizma u bolestima pankreasa i uglavnom su dale oprečne rezultate. Značaj GSTP1 Ile105Val polimorfizma za bolesti pankreasa još uvek nije ra