Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins

The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate time-lapse imaging distinct phenotypes of prostate organoids derived from patien...

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Bibliographic Details
Published in:Frontiers in oncology 2023-01, Vol.12, p.1083150-1083150
Main Authors: Marr, Kendra D, Ignatenko, Natalia A, Warfel, Noel A, Batai, Ken, Cress, Anne E, Pollock, Grant R, Wong, Ava C, Lee, Benjamin R
Format: Article
Language:English
Subjects:
cancer phenotypes
extracellular matrix
Oncology
organoids
prostate cancer
video microscopy
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Summary:The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate time-lapse imaging distinct phenotypes of prostate organoids derived from patient material- without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p
ISSN:2234-943X
2234-943X
DOI:10.3389/fonc.2022.1083150