High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers

The increasing prevalence of multi-drug resistant (MDR) Escherichia coli in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant E. coli isolates from Arapirama gigas, a major fish s...

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Published in:Microorganisms (Basel) 2022-04, Vol.10 (4), p.808
Main Authors: Lima, Luciana Sampaio, Proietti-Junior, Aldo Aparecido, Rodrigues, Yan Corrêa, da Silva Vieira, Marcelo Cleyton, Lima, Luana Nepomuceno Gondim Costa, de Oliveira Souza, Cintya, Dias Gonçalves, Verônica, de Oliveira Lima, Marcelo, dos Prazeres Rodrigues, Dália, Lima, Karla Valéria Batista
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Aquaculture
Aquatic ecosystems
Automation
Data points
Dehydrogenases
DNA polymerase
Drug resistance
E coli
Ecological niches
Enzymes
Epidemiology
ESBLs
Escherichia coli
Farms
Fish
Fish farms
Food sources
Genes
Genetic diversity
Genotyping
Laboratories
molecular epidemiology
Multidrug resistance
PMQR
quinolone-resistance
Reservoirs
Rivers
Strains (organisms)
Virulence
Wildlife
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Summary:The increasing prevalence of multi-drug resistant (MDR) Escherichia coli in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant E. coli isolates from Arapirama gigas, a major fish species in the Brazilian Amazon rivers and fish farms, were characterized regarding their antimicrobial susceptibility, virulence, and genetic diversity. A total of forty (40) specimens of A. gigas, including 20 farmed and 20 wild fish, were included. Thirty-four quinolone-resistant E. coli isolates were phenotypically tested by broth microdilution, while resistance and virulence genes were detected by PCR. Molecular epidemiology and genetic relatedness were analyzed by MLST and PFGE typing. The majority of isolates were classified as MDR and detected harboring blaCTX-M, qnrA and qnrB genes. Enterotoxigenic E. coli pathotype (ETEC) isolates were presented in low prevalence among farmed animals. MLST and PFGE genotyping revealed a wide genetic background, including the detection of internationally spread clones. The obtained data point out A. gigas as a reservoir in Brazilian Amazon aquatic ecosystems and warns of the interference of AMR strains in wildlife and environmental matrices.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10040808