Fluorescent Probes for Super-Resolution Microscopy of Lysosomes

Lysosomes are membrane-enclosed small spherical cytoplasmic organelles. Malfunctioning and abnormalities in lysosomes can cause a plethora of neurodegenerative diseases. Consequently, understanding the structural information on lysosomes down to a subnanometer level is essential. Recently, super-res...

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Bibliographic Details
Published in:ACS omega 2020-10, Vol.5 (42), p.26967-26977
Main Authors: Yadav, Aditya, Rao, Chethana, Nandi, Chayan K
Format: Article
Language:English
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Mini-Review
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Summary:Lysosomes are membrane-enclosed small spherical cytoplasmic organelles. Malfunctioning and abnormalities in lysosomes can cause a plethora of neurodegenerative diseases. Consequently, understanding the structural information on lysosomes down to a subnanometer level is essential. Recently, super-resolution imaging techniques enable us to visualize dynamical processes occurring in suborganelle structures inside living cells down to subnanometer accuracy by breaking the diffraction limit. A brighter and highly photostable fluorescent probe is essential for super-resolution microscopy. In this regard, this mini-review deals with the various types of super-resolution techniques and the probes that are used to specifically stain and resolve the structure of the lysosomes.
ISSN:2470-1343
2470-1343
DOI:10.1021/acsomega.0c04018