Comparison of non-magnetic and magnetic beads multiplex assay for assessment of Plasmodium falciparum antibodies

New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead reagents for cytometric bead assays (CBA...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2019-01, Vol.7, p.e6120-e6120, Article e6120
Main Authors: Ondigo, Bartholomew N, Park, Gregory S, Ayieko, Cyrus, Nyangahu, Donald D, Wasswa, Ronald, John, Chandy C
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Bio-Plex
Disease
Erythrocytes
Global Health
Immunoglobulins
Immunology
Infectious Diseases
Laboratories
Magnetic
Malaria
Medical research
Microspheres
Multiplex
Non-magnetic
Plasmodium falciparum
Plasmodium falciparum antigens
Research centers
Serology
Translational Medicine
Vaccines
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Summary:New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead reagents for cytometric bead assays (CBA) but relative performances are not well published. We have compared two types of bead-based multiplex assays to measure relative antibody levels to malarial antigens. Assays for the measurement of antibodies to five vaccine candidates using non-magnetic and magnetic fluorescent microspheres were compared for their performances with a Bio-Plex  instrument. Mean fluorescence intensity (MFI) was determined from individuals from western Kenya and compared to known positive and negative control plasma samples. recombinant antigens were successfully coupled to both non-magnetic and magnetic beads in multiplex assays. MFIs between the two bead types were comparable for all antigens tested. Bead recovery was superior with magnetic beads for all antigens. MFI values of stored non-magnetic coupled beads did not differ from freshly coupled beads, though they showed higher levels of bead aggregation. Magnetic and non-magnetic beads performed similarly in antibody assays. Magnetic beads were more expensive, but had higher bead recovery, were more convenient to use, and provided rapid and easy protocol manipulation. Magnetic beads are a suitable alternative to non-magnetic beads in malarial antibody serology.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.6120