Composite Hydrogel Microspheres Encapsulating Hollow Mesoporous Imprinted Nanoparticles for Selective Capture and Separation of 2′-Deoxyadenosine

Hollow mesoporous silica nanoparticles have been widely applied as a carrier material in the molecular imprinting process because of their excellent properties, with high specific surface area and well-defined active centers. However, these kinds of materials face the inevitable problem that they ha...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-11, Vol.27 (21), p.7444
Main Authors: Liu, Lu, Zhou, Mengdie, Pan, Jianming
Format: Article
Language:English
Subjects:
2’-deoxyadenosine
Adsorbents
Adsorption
By products
Chemical synthesis
Chemisorption
Deoxyadenosine
Deoxyguanosine
Encapsulation
Ethanol
Genetic engineering
Genetically modified organisms
hollow mesoporous nanoparticles
hydrogel microspheres
Hydrogels
Imprinted polymers
Microspheres
Molecular imprinting
molecularly imprinted polymers
Nanoparticles
Nucleic acids
Polymerization
Polymers
Potassium
selective adsorption
Selectivity
Silica
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Summary:Hollow mesoporous silica nanoparticles have been widely applied as a carrier material in the molecular imprinting process because of their excellent properties, with high specific surface area and well-defined active centers. However, these kinds of materials face the inevitable problem that they have low mass transfer efficiency and cannot be conveniently recycled. In order to solve this problem, this work has developed a composite hydrogel microsphere (MMHSG) encapsulated with hollow mesoporous imprinted nanoparticles for the selective extraction of 2’-deoxyadenosine (dA). Subsequently, the hollow mesoporous imprinted polymers using dA as template molecule and synthesized 5-(2-carbomethoxyvinyl)-2′-deoxyuridine (AcrU) as functional monomer were encapsulated in hydrogel. MMHSG displayed good performance in specifically recognizing and quickly separating dA, whereas no imprinting effect was observed among 2′-deoxyguanosine (dG), deoxycytidine (dC), or 5′-monophosphate disodium salt (AMP). Moreover, the adsorption of dA by MMHSG followed chemisorption and could reach adsorption equilibrium within 60 min; the saturation adsorption capacity was 20.22 μmol·g−1. The introduction of AcrU could improve selectivity through base complementary pairing to greatly increase the imprinting factor to 3.79. Therefore, this was a successful attempt to combine a hydrogel with hollow mesoporous silica nanoparticles and molecularly imprinted material.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27217444