Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

• Published in: International journal of molecular sciences 2020-03, Vol.21 (6), p.2062
• Main Authors: Wójcik-Jagła, Magdalena, Rapacz, Marcin, Dubas, Ewa, Krzewska, Monika, Kopeć, Przemysław, Nowicka, Anna, Ostrowska, Agnieszka, Malaga, Sabina, Żur, Iwona
• Format: Article
• Language: English
• Subjects: Abiotic stress; Actin; Adaptation, Biological; Alpha iron; Barley; candidate genes; Cold; Cold tolerance; Cytochrome; doubled haploid lines; Drought; Drought resistance; drought tolerance; Droughts; Elongation; Environmental stress; Ferredoxin; Ferredoxin-NADP reductase; Freezing; freezing tolerance; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genomes; Genomics; Haploidy; Hordeum - physiology; Invertase; NADP; Open Reading Frames; Population studies; Productivity; Proteins; Proteome; Proteomes; Proteomics - methods; Reductases; Serine; Serine proteinase; Stress, Physiological; Transketolase; Triose-phosphate isomerase
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21062062

Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha ( ), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region ( ), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.