N-OH-AABP Modifications in Human DNA May Lead to Auto-Antibodies in Bladder Cancer Subjects

4-Aminobiphenyl (4-ABP) and other related arylamines have emerged to be responsible for human urinary bladder tumors and cancers. Hemoglobin-ABP adducts have been recognized in the blood of smokers, and it builds up in the circulatory system over the period of years that might lead to a bladder tumo...

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Bibliographic Details
Published in:Diagnostics (Basel) 2022-01, Vol.12 (2), p.337
Main Authors: Shahab, Uzma, Habib, Safia, Alsulimani, Ahmad, Alshammari, Qurain Turki, Alatar, Abdulrahman A, Haque, Shafiul, Uddin, Moin, Ahmad, Saheem
Format: Article
Language:English
Subjects:
4-aminobiphenyl (4-ABP)
Alcohol
Antibodies
Antigens
Bladder cancer
carcinogen
DNA
Genetic testing
Hemoglobin
N-hydroxy-Acetyl 4-Aminobiphenyl (N-OH-AABP)
Sodium
Spectrum analysis
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Summary:4-Aminobiphenyl (4-ABP) and other related arylamines have emerged to be responsible for human urinary bladder tumors and cancers. Hemoglobin-ABP adducts have been recognized in the blood of smokers, and it builds up in the circulatory system over the period of years that might lead to a bladder tumor. N-hydroxy-Acetyl 4-Aminobiphenyl (N-OH-AABP) is one of the reactive forms of 4-ABP which has a potential to initiate tumor growth and causes cancer rapidly. In the present study, commercially available human DNA was modified by N-OH-AABP, and its modifications were analyzed biophysically from fluorescence spectroscopy and thermal denaturation studies. Further, Sera and IgG from bladder cancer patients' blood were assessed for affinity to native and N-OH-AABP modified human DNA using ELISA. The study showed N-OH-AABP caused damage in the structure of the DNA macromolecule and the perturbations resulting from damage leads to change in the Tm of the DNA molecule. Bladder cancer auto-antibodies, particularly in smoker group, showed preferential binding to N-OH-AABP modified human DNA. This study shows that N-OH-AABP modified DNA could be an antigenic stimulus for the generation of autoantibodies in the sera of bladder cancer patients.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12020337