Comparability of hemoglobin concentration measurements from the HemoCue Hb201+ and Hb801 in birds and a mammal with and without nucleated red-blood cells

HemoCue analyzers are standard point-of-care devices for measuring blood hemoglobin concentration ([Hb]). The utility of these photometers has been demonstrated for all vertebrate lineages including birds, yet because most vertebrates except mammals possess nucleated red-blood cells, their [Hb] read...

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Bibliographic Details
Published in:Journal of field ornithology 2024-09, Vol.95 (3), p.5, Article art5
Main Authors: McCracken, Kevin, Johnston, Haley, McCracken, Katherine, Olson, Riley, Otero Soberon, Jose Antonio, Turpo Aroquipa, Renzo Hernan, Alza, Luis
Format: Article
Language:English
Subjects:
avian hematology
hemocue
hemoglobin concentration
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Summary:HemoCue analyzers are standard point-of-care devices for measuring blood hemoglobin concentration ([Hb]). The utility of these photometers has been demonstrated for all vertebrate lineages including birds, yet because most vertebrates except mammals possess nucleated red-blood cells, their [Hb] readings are inflated upward in these taxa and require a correction factor relative to the reference calibration. We field tested the newest of these analyzers, Hb801, against its predecessor, the Hb201+. Because of differences in the photometry methods, these two analyzers were not found to be equivalent for taxa with nucleated red-blood cells, such as birds. Whereas a correction factor of 1.0–1.4 g/dL is required for the Hb201+, it may be that no correction factor is required for the Hb801. Further study of the Hb801 relative to a gold-standard reference in non-mammalian lineages is warranted.
ISSN:1557-9263
1557-9263
DOI:10.5751/JFO-00493-950305