Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity

The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis. Thirty male Wistar rats were randomly divided into five groups of 6...

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Bibliographic Details
Published in:Iranian journal of basic medical sciences 2020-05, Vol.23 (5), p.569-575
Main Authors: Ghorbani-Taherdehi, Faezeh, Nikravesh, Mohammad Reza, Jalali, Mehdi, Fazel, Alireza, Gorji-Valokola, Mahmoud
Format: Article
Language:English
Subjects:
Acids
Apoptosis
ascorbic acid
malathion
Original
Oxidation
pcna
rat
tunel technique
Vitamin C
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Summary:The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis. Thirty male Wistar rats were randomly divided into five groups of 6 rats each, including control (no intervention), sham (normal saline 0.09%), malathion (50 mg/kg), malathion plus ascorbic acid (50 mg/kg and 200 mg/kg, respectively), and ascorbic acid (200 mg/kg) groups. Malathion and ascorbic acid were administrated via intraperitoneal injection once per day and seven times per week. After 6 weeks, animals were sacrificed, and testis tissue was used for evaluation of apoptosis and proliferation of germinal epithelium cells using the TUNEL and PCNA staining techniques. The results of TUNEL staining showed that the numbers of apoptotic cells in spermatogonia and primary spermatocyte cells were significantly increased in the malathion 50 mg/kg group vs control group (
ISSN:2008-3866
2008-3874
DOI:10.22038/ijbms.2020.35952.8608