Potential Role of EPSPS Mutations in the Resistance of Eleusine indica to Glyphosate

Gene mutation is a basic evolutionary mechanism in plants under selection pressure of herbicides. Such mutation has pleiotropic effects on plant growth. We systemically investigated the effects of Pro106Leu (P106L), Pro106Ser (P106S), and Thr102Ile + Pro106Ser (TIPS) mutations on EPSPS functionality...

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Published in:International journal of molecular sciences 2023-05, Vol.24 (9), p.8250
Main Authors: Chen, Jingchao, Cui, Hailan, Li, Zhiling, Yu, Haiyan, Hou, Qiang, Li, Xiangju
Format: Article
Language:English
Subjects:
3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics
affinity
E coli
Eleusine - genetics
Eleusine - metabolism
Eleusine indica
Enzymes
fitness
Gene Expression Regulation, Plant
Genes
Glyphosate
Herbicide Resistance - genetics
Herbicides
Herbicides - metabolism
Herbicides - pharmacology
Leaf area
Metabolites
metabolomics
Mutants
Mutation
Nucleotides
Physiology
Point mutation
Seedlings
Weeds
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Summary:Gene mutation is a basic evolutionary mechanism in plants under selection pressure of herbicides. Such mutation has pleiotropic effects on plant growth. We systemically investigated the effects of Pro106Leu (P106L), Pro106Ser (P106S), and Thr102Ile + Pro106Ser (TIPS) mutations on EPSPS functionality and fitness traits in at the biochemical and physiological levels. The affinity of natural EPSPS for glyphosate was 53.8 times higher than that for phosphoenolpyruvate (PEP), as revealed by the dissociation constant; the constant decreased in both the P106L (39.9-fold) and P106S (46.9-fold) mutants but increased in the TIPS (87.5-fold) mutant. The (PEP) values of the P106L, P106S, and TIPS mutants were 2.4-, 0.7-, and 4.1-fold higher than that of natural EPSPS, corresponding to resistance levels of 2.5, 1.9, and 11.4, respectively. The catalytic efficiency values (maximum reaction rates) were 0.89-, 0.94-, and 0.26-fold higher than that of natural EPSPS. The levels of metabolites related to amino acids and nucleotides were significantly reduced in the mutated plants. The fitness costs were substantial for the biomass, total leaf area, seed number, and seedling emergence throughout the growth period in the plants with P106L and TIPS mutations. These results provide insights into EPSPS kinetics and their effect on plant growth.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24098250