Single-cell analysis reveals congruence between kidney organoids and human fetal kidney

Human kidney organoids hold promise for studying development, disease modelling and drug screening. However, the utility of stem cell-derived kidney tissues will depend on how faithfully these replicate normal fetal development at the level of cellular identity and complexity. Here, we present an in...

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Bibliographic Details
Published in:Genome medicine 2019-01, Vol.11 (1), p.3-3, Article 3
Main Authors: Combes, Alexander N, Zappia, Luke, Er, Pei Xuan, Oshlack, Alicia, Little, Melissa H
Format: Article
Language:English
Subjects:
Analysis
Artefacts
Cell differentiation
Cell Line
Cell Lineage
Datasets
Drug evaluation
Drug screening
Endothelium
Fetal development
Fetuses
Gene expression
Glycoproteins - genetics
Glycoproteins - metabolism
Health screening
Human kidney organoids
Humans
Induced pluripotent cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Kidney - cytology
Kidney - embryology
Kidneys
Medical research
Neuronal-glial interactions
Organoids
Organoids - cytology
Organoids - metabolism
Progenitor cells
RNA
RNA sequencing
Single-Cell Analysis
Single-cell RNA sequencing
Stem cell-derived models
Stem cells
Stroma
Transcription
Wnt Proteins - genetics
Wnt Proteins - metabolism
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Summary:Human kidney organoids hold promise for studying development, disease modelling and drug screening. However, the utility of stem cell-derived kidney tissues will depend on how faithfully these replicate normal fetal development at the level of cellular identity and complexity. Here, we present an integrated analysis of single cell datasets from human kidney organoids and human fetal kidney to assess similarities and differences between the component cell types. Clusters in the combined dataset contained cells from both organoid and fetal kidney with transcriptional congruence for key stromal, endothelial and nephron cell type-specific markers. Organoid enriched neural, glial and muscle progenitor populations were also evident. Major transcriptional differences between organoid and human tissue were likely related to technical artefacts. Cell type-specific comparisons revealed differences in stromal, endothelial and nephron progenitor cell types including expression of WNT2B in the human fetal kidney stroma. This study supports the fidelity of kidney organoids as models of the developing kidney and affirms their potential in disease modelling and drug screening.
ISSN:1756-994X
1756-994X
DOI:10.1186/s13073-019-0615-0