Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

The extracellular environment of vascular cells in vivo is complex in its chemical composition, physical properties, and architecture. Consequently, it has been a great challenge to study vascular cell responses in vitro, either to understand their interaction with their native environment or to inv...

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Bibliographic Details
Published in:Beilstein journal of nanotechnology 2016, Vol.7 (1), p.1620-1641
Main Authors: Greiner, Alexandra M, Sales, Adria, Chen, Hao, Biela, Sarah A, Kaufmann, Dieter, Kemkemer, Ralf
Format: Article
Language:English
Subjects:
Adhesives
Biology
Cell adhesion & migration
Chemical composition
Endothelial cells
Endothelium
Extracellular matrix
fabrication methods
Gene expression
Materials science
materials selection
Mechanical properties
Microscopy
Muscles
nano- and micro-topography
Nanoscience
Nanostructure
Nanotechnology
Physical properties
Plasma etching
Polymerization
Review
Silicon wafers
Smooth muscle
Surgical implants
Topography
Transplants & implants
vascular endothelial cells
vascular smooth muscle cells
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Summary:The extracellular environment of vascular cells in vivo is complex in its chemical composition, physical properties, and architecture. Consequently, it has been a great challenge to study vascular cell responses in vitro, either to understand their interaction with their native environment or to investigate their interaction with artificial structures such as implant surfaces. New procedures and techniques from materials science to fabricate bio-scaffolds and surfaces have enabled novel studies of vascular cell responses under well-defined, controllable culture conditions. These advancements are paving the way for a deeper understanding of vascular cell biology and materials-cell interaction. Here, we review previous work focusing on the interaction of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) with materials having micro- and nanostructured surfaces. We summarize fabrication techniques for surface topographies, materials, geometries, biochemical functionalization, and mechanical properties of such materials. Furthermore, various studies on vascular cell behavior and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on potential interesting future studies.
ISSN:2190-4286
2190-4286
DOI:10.3762/bjnano.7.155