Single cell transcriptomics suggest that human adipocyte progenitor cells constitute a homogeneous cell population

Regulation of adipose tissue stem cells (ASCs) and adipogenesis impact the development of excess body fat-related metabolic complications. Animal studies have suggested the presence of distinct subtypes of ASCs with different differentiation properties. In addition, ASCs are becoming the biggest sou...

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Bibliographic Details
Published in:Stem cell research & therapy 2017-11, Vol.8 (1), p.250-250, Article 250
Main Authors: Acosta, Juan R, Joost, Simon, Karlsson, Kasper, Ehrlund, Anna, Li, Xidan, Aouadi, Myriam, Kasper, Maria, Arner, Peter, Rydén, Mikael, Laurencikiene, Jurga
Format: Article
Language:English
Subjects:
Adipocyte progenitor
Adipose tissues
Health aspects
Human adipose tissue
Medicin och hälsovetenskap
Mesenchymal stem cells
Short Report
Single cell sequencing
Stem cells
Transcription factors
Transplantation
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Summary:Regulation of adipose tissue stem cells (ASCs) and adipogenesis impact the development of excess body fat-related metabolic complications. Animal studies have suggested the presence of distinct subtypes of ASCs with different differentiation properties. In addition, ASCs are becoming the biggest source of mesenchymal stem cells used in therapies, which requires deep characterization. Using unbiased single cell transcriptomics we aimed to characterize ASC populations in human subcutaneous white adipose tissue (scWAT). The transcriptomes of 574 single cells from the WAT total stroma vascular fraction (SVF) of four healthy women were analyzed by clustering and t-distributed stochastic neighbor embedding visualization. The identified cell populations were then mapped to cell types present in WAT using data from gene expression microarray profiling of flow cytometry-sorted SVF. Cells clustered into four distinct populations: three adipose tissue-resident macrophage subtypes and one large, homogeneous population of ASCs. While pseudotemporal ordering analysis indicated that the ASCs were in slightly different differentiation stages, the differences in gene expression were small and could not distinguish distinct ASC subtypes. Altogether, in healthy individuals, ASCs seem to constitute a single homogeneous cell population that cannot be subdivided by single cell transcriptomics, suggesting a common origin for human adipocytes in scWAT.
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-017-0701-4