Comparative Ubiquitome Analysis Reveals Deubiquitinating Effects Induced by Wolbachia Infection in Drosophila melanogaster

The endosymbiotic Wolbachia bacteria frequently cause cytoplasmic incompatibility (CI) in their insect hosts, where Wolbachia-infected males cross with uninfected females, leading to no or fewer progenies, indicating a paternal modification by Wolbachia. Recent studies have identified a Wolbachia pr...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-08, Vol.23 (16), p.9459
Main Authors: Zong, Qiong, Mao, Bin, Zhang, Hua-Bao, Wang, Bing, Yu, Wen-Juan, Wang, Zhi-Wei, Wang, Yu-Feng
Format: Article
Language:English
Subjects:
Animals
Bacteria
Cell cycle
Cytoplasm - metabolism
Cytoplasmic incompatibility
deubiquitination
Drosophila melanogaster
Drosophila melanogaster - genetics
Enzymes
Female
Fertility
Fruit flies
Incompatibility
Infections
Insects
Male
male fertility
Male sterility
Males
Metabolic pathways
Metabolism
Metabolites
Peptides
Post-translation
Proteasome Endopeptidase Complex - metabolism
Proteasomes
Protein transport
Proteins
proteomics
RNA transport
Semen
Seminal vesicle
Sperm
Spermatogenesis
Testes
Testis - metabolism
Ubiquitin
Ubiquitination
Wolbachia
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Summary:The endosymbiotic Wolbachia bacteria frequently cause cytoplasmic incompatibility (CI) in their insect hosts, where Wolbachia-infected males cross with uninfected females, leading to no or fewer progenies, indicating a paternal modification by Wolbachia. Recent studies have identified a Wolbachia protein, CidB, containing a DUB (deubiquitylating enzyme) domain, which can be loaded into host sperm nuclei and involved in CI, though the DUB activity is not necessary for CI in Drosophila melanogaster. To investigate whether and how Wolbachia affect protein ubiquitination in testes of male hosts and are thus involved in male fertility, we compared the protein and ubiquitinated protein expressions in D. melanogaster testes with and without Wolbachia. A total of 643 differentially expressed proteins (DEPs) and 309 differentially expressed ubiquitinated proteins (DEUPs) were identified to have at least a 1.5-fold change with a p-value of
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23169459