Probable Role of Bilophila wadsworthia in Appendicle Infection

ABSTRACT            Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can...

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Bibliographic Details
Published in:Medical laboratory journal 2020-01, Vol.14 (1), p.29-35
Main Authors: Tabatabaei, Mohammad, Dehvari, Aslam, Geramizadeh, Bita, Niakan, Mohammad Hadi
Format: Article
Language:English
Subjects:
appendectomy
appendicle specimens
bilophila spp
nucleotide sequencing
pcr
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Summary:ABSTRACT            Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens.            Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers.            Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.            Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues.            Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing
ISSN:2538-4449
DOI:10.29252/mlj.14.1.29