Genetic and Probiotic Characteristics of Urolithin A Producing Enterococcus faecium FUA027

FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of FUA027 were evaluated through whole-genome sequence analysis and phenotypic assays. The chromosome s...

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Bibliographic Details
Published in:Foods 2023-02, Vol.12 (5), p.1021
Main Authors: Xia, Mengjie, Mu, Shuting, Fang, Yaowei, Zhang, Xiaomeng, Yang, Guang, Hou, Xiaoyue, He, Fuxiang, Zhao, Yaling, Huang, Yichen, Zhang, Wei, Shen, Juan, Liu, Shu
Format: Article
Language:English
Subjects:
Amino acids
Annotations
Antibiotic resistance
Antibiotics
Antimicrobial agents
Chromatography
Chromosomes
Drug resistance
E coli
Ellagic acid
Enterococcus faecium
Experiments
Fermentation
Food science
Genes
genome
Genomes
Metabolism
Metabolites
Nitrates
Nucleotide sequence
phenotypic assays
Plasmids
Probiotics
Quality control
safety assessment
Sequence analysis
Software
urolithin A
Virulence
Virulence factors
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Summary:FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of FUA027 were evaluated through whole-genome sequence analysis and phenotypic assays. The chromosome size of this strain was 2,718,096 bp, with a GC content of 38.27%. The whole-genome analysis revealed that the genome contained 18 antibiotic resistance genes and seven putative virulence factor genes. FUA027 does not contain plasmids and mobile genetic elements (MGEs), and so the transmissibility of antibiotic resistance genes or putative virulence factors should not occur. Phenotypic testing further indicated that FUA027 is sensitive to clinically relevant antibiotics. In addition, this bacterium exhibited no hemolytic activity, no biogenic amine production, and could significantly inhibit the growth of the quality control strain. In vitro viability was >60% in all simulated gastrointestinal environments, with good antioxidant activity. The study results suggest that FUA027 has the potential to be used in industrial fermentation for the production of urolithin A.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods12051021