Response of seed yield and biochemical traits of Eruca sativa Mill. to drought stress in a collection study

• Published in: Scientific reports 2023-07, Vol.13 (1), p.11157-16, Article 11157
• Main Authors: Nikzad, Sharifeh, Mirmohammady Maibody, Seyed Ali Mohammad, Ehtemam, Mohammad Hossein, Golkar, Pooran, Mohammadi, Seyed Abolghasem
• Format: Article
• Language: English
• Subjects: 631/449; 631/45; Acclimatization - genetics; Antioxidants; Ascorbic acid; Brassicaceae; Carotenoids; Catalase; Catalase - genetics; Chlorophyll; Coloring Agents; Correlation analysis; Crop yield; Drought; Drought resistance; Droughts; Enzymatic activity; Flavonoids; Genotypes; Humanities and Social Sciences; L-Ascorbate peroxidase; Malondialdehyde; multidisciplinary; Peroxidase - genetics; Peroxidases; Phenolic compounds; Phenols; Photosynthetic pigments; Pigments; Plant Breeding; Proline; Science; Science (multidisciplinary); Seeds; Seeds - genetics; Water content
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-38028-6

Drought tolerance is a complex trait in plants that involves different biochemical mechanisms. During two years of study (2019–2020), the responses of 64 arugula genotypes to drought stress were evaluated in a randomized complete block design with three replications under field conditions. Several metabolic traits were evaluated, i.e. relative water content, photosynthetic pigments (chlorophyll and carotenoids), proline, malondialdehyde, enzymatic antioxidants (catalase, ascorbate peroxidase, and peroxidase), total phenolic and flavonoid contents and seed yield. On average, the drought stress significantly increased the proline content (24%), catalase (42%), peroxidase (60%) and malondialdehyde activities (116%) over the two years of study. As a result of the drought stress, the seed yield (18%), relative water content (19.5%) and amount of photosynthetic pigments (chlorophyll and carotenoids) dropped significantly. However, the total phenolic and flavonoid contents showed no significant changes. Under drought stress, the highest seed yields were seen in the G 50 , G 57 , G 54 , G 55 and G 60 genotypes, while the lowest value was observed in the G 16 genotype (94 g plant −1 ). According to the findings, when compared to the drought-sensitive genotypes, the drought-tolerant arugula genotypes were marked with higher levels of proline accumulation and antioxidant enzyme activity. Correlation analysis indicated the positive effects of peroxidase, catalase and proline on seed yield under drought conditions. These traits can be considered for the selection of drought-tolerant genotypes in breeding programs.