Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9

Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining plur...

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Bibliographic Details
Published in:Stem cell research 2016-09, Vol.17 (2), p.441-443
Main Authors: Bara, Anne M., Messana, Angelica, Herring, Amanda, Hazelbaker, Dane Z., Eggan, Kevin, Barrett, Lindy E.
Format: Article
Language:English
Subjects:
Base Sequence
Blotting, Western
Cell Line
Co-Repressor Proteins - chemistry
Co-Repressor Proteins - genetics
Co-Repressor Proteins - metabolism
CRISPR-Cas Systems - genetics
Embryoid Bodies - metabolism
Embryoid Bodies - pathology
Heterozygote
Human Embryonic Stem Cells
Humans
Karyotype
Real-Time Polymerase Chain Reaction
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2016.09.008