Cornus macrophylla , the Antibacterial Activity of Organic Leaf Extracts and the Characterization of the More Lipophilic Components by GC/MS

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-05, Vol.25 (10), p.2395
• Main Authors: Akbar, Muhammad, Ali, Usman, Khalil, Tayyaba, Iqbal, Muhammad Sajjad, Amin, Awais, Naeem, Rehan, Nazir, Abdul, Waqas, Hafiz Muhammad, Aslam, Zohaib, Jafri, Faisal Iqbal, Aslam, Nazir, Chohan, Safeer Akbar
• Format: Article
• Language: English
• Subjects: Acetic acid; Anti-Bacterial Agents - chemistry; Antibacterial activity; Antimicrobial agents; Bacteria; Bacteria - drug effects; Chloroform; Chloroform - chemistry; Cornus - chemistry; Cornus macrophylla; Crop diseases; Diabetic retinopathy; Dimethyl sulfoxide; Ethyl acetate; Flavonoids; Gas chromatography; Gas Chromatography-Mass Spectrometry; GC/MS; Lipophilic; Mass spectrometry; Mass spectroscopy; Metabolites; Methanol - chemistry; Microbial Sensitivity Tests; Pathogens; Penicillin; Pesticides; Phytochemicals; Plant diseases; Plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Researchers; Studies; α-amyrin
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25102395

In the present study, the antibacterial activity of was examined. Organic solvent extracts of leaves were prepared using methanol, hexane, chloroform, and ethyl acetate. Antibacterial activity was examined by using a 100 mg/mL extract concentration. Penicillin was kept as a positive control while dimethyl sulfoxide was taken as a negative control. Methanolic extract exhibited a 21.5, 36.3, 25.3, and 23.7 mm inhibition zone diameter (IZD); -hexane showed a 33, 40, 32.8, and 28.7 mm IZD; chloroform showed a 18.8, 29, 22.3, and 21.6 mm IZD; and ethyl acetate showed a 23.5, 30.2, 30, and 22.3 mm IZD against , and respectively. The -hexane extract revealed high antibacterial activity against all bacterial species as compared with methanolic, chloroform, and ethyl acetate extract. Gas Chromatography Mass Spectrometry (GC/MS) analysis of -hexane extract depicted the presence of 55 compounds. Out of these compounds, one compound, identified as α-amyrin (Mol. wt = 426), exhibited the maximum peak area (32.64%), followed by A'-Neogammacer-22(29)-en-3-ol, acetate, (3.beta.,21.beta.)- (Mol. wt = 468) and β-amyrin (Mol. wt = 426) having peak areas of 25.97 and 6.77%, respectively. It was concluded that the antibacterial activity observed during the present investigation may be due to these compounds.