Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification

• Published in: Virology journal 2011-10, Vol.8 (1), p.489-489, Article 489
• Main Authors: Chen, Hao-tai, Zhang, Jie, Liu, Yong-sheng, Liu, Xiang-tao
• Format: Article
• Language: English
• Subjects: Animals; Clinical medicine; Detection; Diagnosis; DNA polymerases; Foot-and-mouth disease; Foot-and-Mouth Disease - diagnosis; Foot-and-Mouth Disease - virology; Foot-and-Mouth Disease Virus - classification; Foot-and-Mouth Disease Virus - isolation & purification; Genetic aspects; Molecular Diagnostic Techniques - methods; Molecular Typing - methods; Nucleic Acid Amplification Techniques - methods; Physiological aspects; Reverse Transcription; Reverse transcription loop-mediated isothermal amplification; Risk factors; RNA, Viral - genetics; Sensitivity and Specificity; Serotype Asia 1; Short Report; Temperature; Time Factors; Viruses
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422x-8-489

A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations.