Inhibitory Effects of Chlorogenic Acid Containing Green Coffee Bean Extract on Lipopolysaccharide-Induced Inflammatory Responses and Progression of Colon Cancer Cell Line

An inflammatory response, related to colorectal cancer (CRC) progression, is a major subsequent result of bacterial infection following CRC surgery and should be of serious concern. Lipopolysaccharide (LPS), from the bacterial membrane, is a vital mediator of this event through binding with a Toll-l...

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Bibliographic Details
Published in:Foods 2023-07, Vol.12 (14), p.2648
Main Authors: Panyathep, Atita, Punturee, Khanittha, Chewonarin, Teera
Format: Article
Language:English
Subjects:
Acetates
Acetic acid
Acids
Bacterial diseases
Bacterial infections
Beans
Chlorogenic acid
Coffee
Colon
Colon cancer
Colorectal cancer
colorectal cancer resection
Colorectal carcinoma
Development and progression
Esters
Ethyl acetate
Flavonoids
Food science
green coffee bean extract
Health aspects
Inactivation
Infections
Inflammation
Inflammatory response
inflammatory responses
lipopolysaccharide
Lipopolysaccharides
Metastases
Metastasis
NF-κB protein
Pathogens
Survival
TLR4 protein
Toll-like receptors
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Summary:An inflammatory response, related to colorectal cancer (CRC) progression, is a major subsequent result of bacterial infection following CRC surgery and should be of serious concern. Lipopolysaccharide (LPS), from the bacterial membrane, is a vital mediator of this event through binding with a Toll-like receptor 4 (TLR4) and activating through NF-κB in CRC. To identify a novel inhibitor of LPS-induced colon cancer cells (SW480), green coffee bean extract (GBE) was investigated. Ethyl acetate insoluble fraction (EIF) was mainly collected from GBE and classified as chlorogenic acid (CGA)-rich fractions. EIF and CGA inhibited TLR4 expression in LPS-induced SW480 cells. However, EIF was more dominant than CGA, via inhibition of expression and secretion of several associated mediators in inflammatory responses and CRC metastasis through NF-κB inactivation, which resulted in the abrogation of CRC migration and invasion. Thus, CGA-rich fraction from GBE can be further developed as an alternative treatment, coupled with CRC surgical treatment, to increase therapeutic efficiency and survival rate.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods12142648