Anopheles gambiae larvae's ability to grow and emerge in water containing lethal concentrations of clothianidin, acetamiprid, or imidacloprid is consistent with cross-resistance to neonicotinoids

• Published in: Parasites & vectors 2024-03, Vol.17 (1), p.98-12, Article 98
• Main Authors: Ambadiang, Marilene, Fouet, Caroline, Ashu, Fred, Bouaka, Calmes, Penlap-Beng, Véronique, Kamdem, Colince
• Format: Article
• Language: English
• Subjects: Agriculture; Agrochemicals; Animals; Anopheles; Anopheles coluzzii; Anopheles gambiae; Aquatic habitats; Aquatic insects; Cameroon - epidemiology; Chlorfenapyr; Control; Corrosion resistance; Cross-resistance; Culicidae; Deltamethrin; Effectiveness; Enzymes; Growth; Guanidines; Imidacloprid; Insect control; Insecticide Resistance; Insecticides; Insecticides - pharmacology; Insects; Instars; Larva; Larvae; Lethal limits; Malaria; Metabolism; Methods; Mortality; Mosquito Vectors; Mosquitoes; Neonicotinoid insecticides; Neonicotinoids; Neonicotinoids - pharmacology; Nitriles; Nitro Compounds; Pesticide residues; Pesticides; Physiological aspects; Populations; Prevention; Public health; Pyrethrins; Pyrethroids; Resistance mechanisms; Risk factors; Sibling species; Sublethal effects; Suburban areas; Survival; Thiazoles; Urbanization; Vector control; Water
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-024-06188-7

For decades, various agrochemicals have been successfully repurposed for mosquito control. However, preexisting resistance caused in larval and adult populations by unintentional pesticide exposure or other cross-resistance mechanisms poses a challenge to the efficacy of this strategy. A better understanding of larval adaptation to the lethal and sublethal effects of residual pesticides in aquatic habitats would provide vital information for assessing the efficacy of repurposed agrochemicals against mosquitoes. We reared field-collected mosquito larvae in water containing a concentration of agrochemical causing 100% mortality in susceptible mosquitoes after 24 h (lethal concentration). Using this experimental setup, we tested the effect of lethal concentrations of a pyrrole (chlorfenapyr, 0.10 mg/l), a pyrethroid (deltamethrin, 1.5 mg/l), and three neonicotinoids including imidacloprid (0.075 mg/l), acetamiprid (0.15 mg/l), and clothianidin (0.035 mg/l) on mortality rates, growth, and survival in third-instar larvae of the two sibling species Anopheles gambiae and Anopheles coluzzii collected from Yaoundé, Cameroon. We found that An. gambiae and An. coluzzii larvae were susceptible to chlorfenapyr and were killed within 24 h by a nominal concentration of 0.10 mg/l. Consistent with strong resistance, deltamethrin induced low mortality in both species. Lethal concentrations of acetamiprid, imidacloprid, and clothianidin strongly inhibited survival, growth, and emergence in An. coluzzii larvae. By contrast, depending on the active ingredient and the population tested, 5-60% of immature stages of An. gambiae were able to grow and emerge in water containing a lethal concentration of neonicotinoids, suggesting cross-resistance to this class of insecticides. These findings corroborate susceptibility profiles observed in adults and suggest that unintentional pesticide exposure or other cross-resistance processes could contribute to the development of resistance to neonicotinoids in some Anopheles populations.