In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes

Microvascular disease is frequently found in major pathologies affecting vital organs, such as the brain, heart, and kidneys. While imaging modalities, such as ultrasound, computed tomography, single photon emission computed tomography, and magnetic resonance imaging, are widely used to visualize va...

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Bibliographic Details
Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2022-02, Vol.15 (3), p.292
Main Authors: Wang, Shaowei, Budzevich, Mikalai, Abdalah, Mahmoud A, Balagurunathan, Yoganand, Choi, Jung W
Format: Article
Language:English
Subjects:
Cardiovascular disease
Contrast agents
Coronary vessels
Defects
Diabetes
Electrocardiography
FDG
Glucose
Heart attacks
Magnetic resonance imaging
Metabolism
Metabolites
microvasculature imaging
PET/CT
Rodents
Scanners
Tomography
vascular imaging
Vein & artery diseases
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Summary:Microvascular disease is frequently found in major pathologies affecting vital organs, such as the brain, heart, and kidneys. While imaging modalities, such as ultrasound, computed tomography, single photon emission computed tomography, and magnetic resonance imaging, are widely used to visualize vascular abnormalities, the ability to non-invasively assess an organ's total vasculature, including microvasculature, is often limited or cumbersome. Previously, we have demonstrated proof of concept that non-invasive imaging of the total mouse vasculature can be achieved with 18F-fluorodeoxyglucose (18F-FDG)-labeled human erythrocytes and positron emission tomography/computerized tomography (PET/CT). In this work, we demonstrate that changes in the total vascular volume of the brain and left ventricular myocardium of normal rats can be seen after pharmacological vasodilation using 18F-FDG-labeled rat red blood cells (FDG RBCs) and microPET/CT imaging. FDG RBC PET imaging was also used to approximate the location of myocardial injury in a surgical myocardial infarction rat model. Finally, we show that FDG RBC PET imaging can detect relative differences in the degree of drug-induced intra-myocardial vasodilation between diabetic rats and normal controls. This FDG-labeled RBC PET imaging technique may thus be useful for assessing microvascular disease pathologies and characterizing pharmacological responses in the vascular bed of interest.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph15030292