Parallel Processing of Sound Dynamics across Mouse Auditory Cortex via Spatially Patterned Thalamic Inputs and Distinct Areal Intracortical Circuits

• Published in: Cell reports (Cambridge) 2019-04, Vol.27 (3), p.872-885.e7
• Main Authors: Liu, Ji, Whiteway, Matthew R., Sheikhattar, Alireza, Butts, Daniel A., Babadi, Behtash, Kanold, Patrick O.
• Format: Article
• Language: English
• Subjects: auditory cortex; MGB; mouse; pattern; temporal; two-photon imaging
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.03.069

Natural sounds have rich spectrotemporal dynamics. Spectral information is spatially represented in the auditory cortex (ACX) via large-scale maps. However, the representation of temporal information, e.g., sound offset, is unclear. We perform multiscale imaging of neuronal and thalamic activity evoked by sound onset and offset in awake mouse ACX. ACX areas differed in onset responses (On-Rs) and offset responses (Off-Rs). Most excitatory L2/3 neurons show either On-Rs or Off-Rs, and ACX areas are characterized by differing fractions of On and Off-R neurons. Somatostatin and parvalbumin interneurons show distinct temporal dynamics, potentially amplifying Off-Rs. Functional network analysis shows that ACX areas contain distinct parallel onset and offset networks. Thalamic (MGB) terminals show either On-Rs or Off-Rs, indicating a thalamic origin of On and Off-R pathways. Thus, ACX areas spatially represent temporal features, and this representation is created by spatial convergence and co-activation of distinct MGB inputs and is refined by specific intracortical connectivity. [Display omitted] •Automatic image segmentation defines auditory cortex fields by temporal responses•Both onset and offset responses are tonotopically organized across auditory cortex•Parallel neuronal networks process tone onset and offset in the auditory cortex•A1 amplifies offset response by convergent thalamic input and intracortical processing Using multiscale imaging of mouse auditory cortices, Liu et al. found that the offset response is tonotopically organized and spatially extensive across auditory fields, while A1 L2/3 pyramidal neurons process tone onset and offset in parallel networks. The offset response is amplified by differential convergence of thalamic input and intracortical processing involving interneurons.