Dietary butyrate ameliorates metabolic health associated with selective proliferation of gut Lachnospiraceae bacterium 28-4

Short-chain fatty acids, including butyrate, have multiple metabolic benefits in individuals who are lean but not in individuals with metabolic syndrome, with the underlying mechanisms still being unclear. We aimed to investigate the role of gut microbiota in the induction of metabolic benefits of d...

Full description

Saved in:
Bibliographic Details
Published in:JCI insight 2023-02, Vol.8 (4)
Main Authors: Li, Zhuang, Zhou, Enchen, Liu, Cong, Wicks, Hope, Yildiz, Sena, Razack, Farhana, Ying, Zhixiong, Kooijman, Sander, Koonen, Debby PY, Heijink, Marieke, Kostidis, Sarantos, Giera, Martin, Sanders, Ingrid MJG, Kuijper, Ed J, Smits, Wiep Klaas, van Dijk, Ko Willems, Rensen, Patrick CN, Wang, Yanan
Format: Article
Language:English
Subjects:
Animals
Butyrates - adverse effects
Cell Proliferation
Endocrinology
Humans
Metabolic Syndrome
Mice
Microbiology
Obesity - metabolism
RNA, Ribosomal, 16S
Weight Gain
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Short-chain fatty acids, including butyrate, have multiple metabolic benefits in individuals who are lean but not in individuals with metabolic syndrome, with the underlying mechanisms still being unclear. We aimed to investigate the role of gut microbiota in the induction of metabolic benefits of dietary butyrate. We performed antibiotic-induced microbiota depletion of the gut and fecal microbiota transplantation (FMT) in APOE*3-Leiden.CETP mice, a well-established translational model for developing human-like metabolic syndrome, and revealed that dietary butyrate reduced appetite and ameliorated high-fat diet-induced (HFD-induced) weight gain dependent on the presence of gut microbiota. FMT from butyrate-treated lean donor mice, but not butyrate-treated obese donor mice, into gut microbiota-depleted recipient mice reduced food intake, attenuated HFD-induced weight gain, and improved insulin resistance. 16S rRNA and metagenomic sequencing on cecal bacterial DNA of recipient mice implied that these effects were accompanied by the selective proliferation of Lachnospiraceae bacterium 28-4 in the gut as induced by butyrate. Collectively, our findings reveal a crucial role of gut microbiota in the beneficial metabolic effects of dietary butyrate as strongly associated with the abundance of Lachnospiraceae bacterium 28-4.
ISSN:2379-3708
2379-3708
DOI:10.1172/jci.insight.166655