Gastroprotective effects and metabolomic profiling of Chasteberry fruits against indomethacin-induced gastric injury in rats

• Published in: Journal of functional foods 2021-11, Vol.86, p.104732, Article 104732
• Main Authors: Ogaly, Hanan A., Alsherbiny, Muhammad A., El Badawy, Shymaa A., Abd-Elsalam, Reham M., Guang Li, Chun, Azouz, Asmaa A.
• Format: Article
• Language: English
• Subjects: Apoptosis; Gastric injury; Indomethacin; Metabolomic profiling; NF-κB signalling; UPLC-QTOF-MS; Vitex agnus castus
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2021.104732

[Display omitted] •This study is the first to highlight the potential gastro-protective activity of Vitex agnus castus extract VACE, the effect may be related to its arsenal metabolites.•VACE (120 mg/kg) showed a comparable protection as esomeprazole in gastroprotection scoring, antioxidative and anti-apoptotic activity-related parameters.•Antioxidant, anti-inflammatory, and regulation of NF-κß and apoptosis pathways are among the mechanisms of VACE-induced protective activity.•This beneficial effects of VACE suggested it as a promising natural anti-ulcerogenic supplement. Vitex agnus castus L. extract (VACE) was investigated for its gastroprotective properties and possible molecular mechanisms in rats. VACE (60 or 120 mg/kg) or Esomeprazole (20 mg/kg) were orally administered for 3 weeks before the induction of gastropathy using indomethacin (30 mg/kg, single oral dose). VACE ameliorated the indomethacin-induced gastric juice acidity and pathological changes. VACE significantly preserved GSH, SOD, NO and PGE2 contents, while decreased lipid-peroxide, TNF-α and MPO contents. Moreover, VACE downregulated NF-κB1, COX-2, Caspase-3 and upregulated Bcl-2 and HSP-70 expression. Ultra-high-performance liquid chromatography (UPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (qTOF-MS) enabled the tentative identification of 87 compounds allocated in seven main classes including flavonoids, glycosylated iridoid and labdane diterpenes. Notably, different agnuside derivatives and diterpenoids were reported in VACE for the first time. In conclusion, VACE contains an arsenal of bioactive metabolites which may exhibit gastroprotection by inhibiting inflammation, oxidative stress, and apoptosis.