A novel strategy to characterize the pattern of β-lactam antibiotic-induced drug resistance in Acinetobacter baumannii

Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent public health threat, according to the CDC. This pathogen has few treatment options and causes severe nosocomial infections with > 50% fatality rate. Although previous studies have examined the proteome of CRAb, there have been no f...

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Bibliographic Details
Published in:Scientific reports 2023-06, Vol.13 (1), p.9177-9177, Article 9177
Main Authors: Hillyer, Trae, Benin, Bogdan M., Sun, Chuanqi, Aguirre, Noah, Willard, Belinda, Sham, Yuk Yin, Shin, Woo Shik
Format: Article
Language:English
Subjects:
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Acinetobacter baumannii
Amoxicillin
Antibiotics
Drug resistance
Health risks
Humanities and Social Sciences
Isoforms
multidisciplinary
Multidrug resistance
Nosocomial infection
Penicillin
Proteomes
Proteomics
Public health
Science
Science (multidisciplinary)
Trypsin
β Lactamase
β-Lactam antibiotics
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Summary:Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent public health threat, according to the CDC. This pathogen has few treatment options and causes severe nosocomial infections with > 50% fatality rate. Although previous studies have examined the proteome of CRAb, there have been no focused analyses of dynamic changes to β-lactamase expression that may occur due to drug exposure. Here, we present our initial proteomic study of variation in β-lactamase expression that occurs in CRAb with different β-lactam antibiotics. Briefly, drug resistance to Ab (ATCC 19606) was induced by the administration of various classes of β-lactam antibiotics, and the cell-free supernatant was isolated, concentrated, separated by SDS-PAGE, digested with trypsin, and identified by label-free LC–MS-based quantitative proteomics. Thirteen proteins were identified and evaluated using a 1789 sequence database of Ab β-lactamases from UniProt, the majority of which were Class C β-lactamases (≥ 80%). Importantly, different antibiotics, even those of the same class (e.g. penicillin and amoxicillin), induced non-equivalent responses comprising various isoforms of Class C and D serine-β-lactamases, resulting in unique resistomes. These results open the door to a new approach of analyzing and studying the problem of multi-drug resistance in bacteria that rely strongly on β-lactamase expression.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-36475-9