Highly sensitive Thin-Layer Chromatographic–Densitometric assay for quantitation of Amisulpride & Zolmitriptan in synthetic mixture: Application to pharmaceutical formulation & biological fluids

• Published in: Results in Chemistry 2021-01, Vol.3, p.100180, Article 100180
• Main Authors: Eltoukhi, Walid E., Atia, Noha N., Marzouq, Mostafa A., Hassan, Ahmed I.
• Format: Article
• Language: English
• Subjects: Amisulpride; Biological fluid; Chromatography; Dosage forms; Zolmitriptan
• ISSN: 2211-7156; 2211-7156
• DOI: 10.1016/j.rechem.2021.100180

•New (TLC) assay was estimated to quantificate of synthetic mixture of (AMS) with (ZOL).•Validation of the proposed method according to the ICH and USP guidelines.•The proposed assay is constructed for quantification of cited drugs in their preparation and human plasma. A new and sensitive (TLC) assay has been estimated for quantitation of synthetic binary mixtures of antipsychotic drug Amisulpride (AMS) with antimigarine drug Zolmitriptan (ZOL). These medications co-administered with each other for treatment of chronic migraine headache associated with psychosis. The synthetic mixture has been separated on precoated silica TLC plates G60 F254 by using mobile phase which consist of mixture of Chloroform: Ethyl acetate: Methanol: Ammonia in ratio (30:30:73:3 v/v/v/v). The mixture has been determined at λmax 231 nm. Retardation factor (RF) values for AMS and ZOL have been 0.6 and 0.43 respectively. Calibration graphs have been linear in range 50–500 ng/spot for all studied drugs. The detection limits have been 6.04 & 10.5 ng/spot and the quantitation limits have been 18.3 & 31.834 ng/spot for AMS and ZOL respectively. The described assay has been utilized for quantitation of synthetic mixture in pharmaceutical tablet &biological fluids.