Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents

The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus limiting their health benefits. Cellulolytic enzyme-assisted extraction (CAE) potentially solves this issue; however, solvent extraction requires reco...

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Bibliographic Details
Published in:Bioresources and bioprocessing 2021-08, Vol.8 (1), p.76-76, Article 76
Main Authors: Makkliang, Fonthip, Siriwarin, Boondaree, Yusakul, Gorawit, Phaisan, Suppalak, Sakdamas, Attapon, Chuphol, Natthapon, Putalun, Waraporn, Sakamoto, Seiichi
Format: Article
Language:English
Subjects:
Aglycones
Biochemical Engineering
Biocompatibility
Biotransformation
CAD
Cellulolytic enzymes
Chemistry
Chemistry and Materials Science
Choline
Computer aided design
Daidzein
Environmental Engineering/Biotechnology
Enzymatic activity
Enzyme activity
Enzymes
Genistein
Glycosides
Hydrophobicity
Industrial and Production Engineering
Isoflavones
Isoflavonoid
Microflora
Natural deep eutectic solvent
Propylene
Propylene glycol
Pueraria
Pueraria mirifica
Response surface methodology
Solvent extraction
Solvents
Transformations
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Summary:The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus limiting their health benefits. Cellulolytic enzyme-assisted extraction (CAE) potentially solves this issue; however, solvent extraction requires recovery of the hydrophobic products. Here, we established the simultaneous transformation and extraction of PMIs using cellulolytic enzymes and natural deep eutectic solvents (NADESs). The NADES compositions were optimized to allow the use of NADESs as CAE media, and the extraction parameters were optimized using response surface methodology (RSM). The optimal conditions were 14.7% (v/v) choline chloride:propylene glycol (1:2 mol ratio, ChCl:PG) at 56.1 °C for the cellulolytic enzyme (262 mU/mL) reaction in which daidzin and genistin were extracted and wholly transformed to their aglycones daidzein and genistein. The extraction of PMIs using ChCl:PG is more efficient than that using conventional solvents; additionally, biocompatible ChCl:PG enhances cellulolytic enzyme activity, catalyzing the transformation of PMIs into compounds with higher estrogenicity and absorbability.
ISSN:2197-4365
2197-4365
DOI:10.1186/s40643-021-00428-9