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Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity. We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3...
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| Published in: | Journal of translational medicine 2019-07, Vol.17 (1), p.232-232, Article 232 |
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| container_title | Journal of translational medicine |
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| creator | Barberio, Matthew D Kasselman, Lora J Playford, Martin P Epstein, Samuel B Renna, Heather A Goldberg, Madeleine DeLeon, Joshua Voloshyna, Iryna Barlev, Ashley Salama, Michael Ferrante, Sarah C Nadler, Evan P Mehta, Nehal Reiss, Allison B Freishtat, Robert J |
| description | Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.
We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m
) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) adipocyte-derived extracellular vesicle microRNAs in serum; (3) the role of visceral adipose tissue extracellular vesicle in regulation of cholesterol efflux and cholesterol efflux gene expression in THP-1 macrophages in vitro.
Efflux capacity was significantly associated with HDL (r = 0.30, p = 0.01) and LDL (r = 0.33, p = 0.005) particle size. Multivariate-analysis identified six microRNAs associated (p |
| doi_str_mv | 10.1186/s12967-019-1980-6 |
| format | article |
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We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m
) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) adipocyte-derived extracellular vesicle microRNAs in serum; (3) the role of visceral adipose tissue extracellular vesicle in regulation of cholesterol efflux and cholesterol efflux gene expression in THP-1 macrophages in vitro.
Efflux capacity was significantly associated with HDL (r = 0.30, p = 0.01) and LDL (r = 0.33, p = 0.005) particle size. Multivariate-analysis identified six microRNAs associated (p < 0.05) with cholesterol efflux capacity: miR-3129-5p (Beta = 0.695), miR-20b (0.430), miR9-5p (0.111), miR-320d (- 0.190), miR301a-5p (0.042), miR-155-5p (0.004). In response to increasing concentrations (1 μg/mL vs. 3 μg/mL) of VAT extracellular vesicle, cholesterol efflux (66% ± 10% vs. 49% ± 2%; p < 0.01) and expression of ABCA1 (FC = 1.9 ± 0.8 vs 0.5 ± 0.2; p < 0.001), CD36 (0.7 ± 0.4 vs. 2.1 ± 0.8, p = 0.02), CYP27A1 (1.4 ± 0.4 vs. 0.9 ± 0.5; p < 0.05), and LXRA (1.8 ± 1.1 vs. 0.5 ± 0.2; p < 0.05) was altered in THP-1 cells in vitro.
Adipocyte-derived extracellular vesicle microRNAs may, in part, be involved macrophage cholesterol efflux regulation.</description><identifier>ISSN: 1479-5876</identifier><identifier>EISSN: 1479-5876</identifier><identifier>DOI: 10.1186/s12967-019-1980-6</identifier><identifier>PMID: 31331347</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adipose tissue ; Adolescent obesity ; Biochemistry ; Cardiovascular diseases ; Care and treatment ; Cholesterol ; Cholesterol Efflux ; Cholesterol metabolism ; Cytochrome P-450 ; Extracellular vesicle ; Gene expression ; Genes ; Genetic aspects ; Health aspects ; Low density lipoproteins ; Macrophages ; Medical research ; MicroRNA ; microRNAs ; Obesity</subject><ispartof>Journal of translational medicine, 2019-07, Vol.17 (1), p.232-232, Article 232</ispartof><rights>COPYRIGHT 2019 BioMed Central Ltd.</rights><rights>The Author(s) 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-3560d149e284b363e3aa127c645e50d6498c13578c80dab80082965e6e69fdae3</citedby><cites>FETCH-LOGICAL-c532t-3560d149e284b363e3aa127c645e50d6498c13578c80dab80082965e6e69fdae3</cites><orcidid>0000-0002-4478-2441</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647309/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647309/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,37013,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31331347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barberio, Matthew D</creatorcontrib><creatorcontrib>Kasselman, Lora J</creatorcontrib><creatorcontrib>Playford, Martin P</creatorcontrib><creatorcontrib>Epstein, Samuel B</creatorcontrib><creatorcontrib>Renna, Heather A</creatorcontrib><creatorcontrib>Goldberg, Madeleine</creatorcontrib><creatorcontrib>DeLeon, Joshua</creatorcontrib><creatorcontrib>Voloshyna, Iryna</creatorcontrib><creatorcontrib>Barlev, Ashley</creatorcontrib><creatorcontrib>Salama, Michael</creatorcontrib><creatorcontrib>Ferrante, Sarah C</creatorcontrib><creatorcontrib>Nadler, Evan P</creatorcontrib><creatorcontrib>Mehta, Nehal</creatorcontrib><creatorcontrib>Reiss, Allison B</creatorcontrib><creatorcontrib>Freishtat, Robert J</creatorcontrib><title>Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs</title><title>Journal of translational medicine</title><addtitle>J Transl Med</addtitle><description>Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.
We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m
) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) adipocyte-derived extracellular vesicle microRNAs in serum; (3) the role of visceral adipose tissue extracellular vesicle in regulation of cholesterol efflux and cholesterol efflux gene expression in THP-1 macrophages in vitro.
Efflux capacity was significantly associated with HDL (r = 0.30, p = 0.01) and LDL (r = 0.33, p = 0.005) particle size. Multivariate-analysis identified six microRNAs associated (p < 0.05) with cholesterol efflux capacity: miR-3129-5p (Beta = 0.695), miR-20b (0.430), miR9-5p (0.111), miR-320d (- 0.190), miR301a-5p (0.042), miR-155-5p (0.004). In response to increasing concentrations (1 μg/mL vs. 3 μg/mL) of VAT extracellular vesicle, cholesterol efflux (66% ± 10% vs. 49% ± 2%; p < 0.01) and expression of ABCA1 (FC = 1.9 ± 0.8 vs 0.5 ± 0.2; p < 0.001), CD36 (0.7 ± 0.4 vs. 2.1 ± 0.8, p = 0.02), CYP27A1 (1.4 ± 0.4 vs. 0.9 ± 0.5; p < 0.05), and LXRA (1.8 ± 1.1 vs. 0.5 ± 0.2; p < 0.05) was altered in THP-1 cells in vitro.
Adipocyte-derived extracellular vesicle microRNAs may, in part, be involved macrophage cholesterol efflux regulation.</description><subject>Adipose tissue</subject><subject>Adolescent obesity</subject><subject>Biochemistry</subject><subject>Cardiovascular diseases</subject><subject>Care and treatment</subject><subject>Cholesterol</subject><subject>Cholesterol Efflux</subject><subject>Cholesterol metabolism</subject><subject>Cytochrome P-450</subject><subject>Extracellular vesicle</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Low density lipoproteins</subject><subject>Macrophages</subject><subject>Medical research</subject><subject>MicroRNA</subject><subject>microRNAs</subject><subject>Obesity</subject><issn>1479-5876</issn><issn>1479-5876</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNptUlFv1SAYbYzGzekP8MWQ-OJLJxRKwQeTmxudSxZNjD4TCh93LLRcob3Z_r3UbstuYiABPs45-TicqnpL8Dkhgn_MpJG8qzGRNZEC1_xZdUpYJ-tWdPz5k_1J9SrnG4wb1jL5sjqhhJbJutNq2F7HAHmCFAMC58J8i3QoRz35OGbkR6TtgjAwTij2kP109wkVNKDoyp3fxwy1heQPYBHcTkkbCGEOOqFDQRsd0OBNij-_b_Lr6oXTIcOb-_Ws-v31y6_tt_rqx8XldnNVm5Y2U01bji1hEhrBesopUK1J0xnOWmix5UwKQ2jbCSOw1b3AWBQjWuDApbMa6Fl1ueraqG_UPvlBpzsVtVf_CjHtlE6TNwEUNyCdc0JSJ5nlRvemaHDQFluLe1a0Pq9a-7kfwC4-JB2ORI9vRn-tdvGgOGcdxbIIfLgXSPHPXLxWg8-LR3qEOGfVNFxQIhspCvT9Ct3p0pofXVzsXOBq00omsGwELajz_6DKsFCcjiM4X-pHBLISyjfknMA9dk-wWpKk1iSpkiS1JEnxwnn39NmPjIfo0L9_AcY4</recordid><startdate>20190722</startdate><enddate>20190722</enddate><creator>Barberio, Matthew D</creator><creator>Kasselman, Lora J</creator><creator>Playford, Martin P</creator><creator>Epstein, Samuel B</creator><creator>Renna, Heather A</creator><creator>Goldberg, Madeleine</creator><creator>DeLeon, Joshua</creator><creator>Voloshyna, Iryna</creator><creator>Barlev, Ashley</creator><creator>Salama, Michael</creator><creator>Ferrante, Sarah C</creator><creator>Nadler, Evan P</creator><creator>Mehta, Nehal</creator><creator>Reiss, Allison B</creator><creator>Freishtat, Robert J</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4478-2441</orcidid></search><sort><creationdate>20190722</creationdate><title>Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs</title><author>Barberio, Matthew D ; Kasselman, Lora J ; Playford, Martin P ; Epstein, Samuel B ; Renna, Heather A ; Goldberg, Madeleine ; DeLeon, Joshua ; Voloshyna, Iryna ; Barlev, Ashley ; Salama, Michael ; Ferrante, Sarah C ; Nadler, Evan P ; Mehta, Nehal ; Reiss, Allison B ; Freishtat, Robert J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-3560d149e284b363e3aa127c645e50d6498c13578c80dab80082965e6e69fdae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adipose tissue</topic><topic>Adolescent obesity</topic><topic>Biochemistry</topic><topic>Cardiovascular diseases</topic><topic>Care and treatment</topic><topic>Cholesterol</topic><topic>Cholesterol Efflux</topic><topic>Cholesterol metabolism</topic><topic>Cytochrome P-450</topic><topic>Extracellular vesicle</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Low density lipoproteins</topic><topic>Macrophages</topic><topic>Medical research</topic><topic>MicroRNA</topic><topic>microRNAs</topic><topic>Obesity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barberio, Matthew D</creatorcontrib><creatorcontrib>Kasselman, Lora J</creatorcontrib><creatorcontrib>Playford, Martin P</creatorcontrib><creatorcontrib>Epstein, Samuel B</creatorcontrib><creatorcontrib>Renna, Heather A</creatorcontrib><creatorcontrib>Goldberg, Madeleine</creatorcontrib><creatorcontrib>DeLeon, Joshua</creatorcontrib><creatorcontrib>Voloshyna, Iryna</creatorcontrib><creatorcontrib>Barlev, Ashley</creatorcontrib><creatorcontrib>Salama, Michael</creatorcontrib><creatorcontrib>Ferrante, Sarah C</creatorcontrib><creatorcontrib>Nadler, Evan P</creatorcontrib><creatorcontrib>Mehta, Nehal</creatorcontrib><creatorcontrib>Reiss, Allison B</creatorcontrib><creatorcontrib>Freishtat, Robert J</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barberio, Matthew D</au><au>Kasselman, Lora J</au><au>Playford, Martin P</au><au>Epstein, Samuel B</au><au>Renna, Heather A</au><au>Goldberg, Madeleine</au><au>DeLeon, Joshua</au><au>Voloshyna, Iryna</au><au>Barlev, Ashley</au><au>Salama, Michael</au><au>Ferrante, Sarah C</au><au>Nadler, Evan P</au><au>Mehta, Nehal</au><au>Reiss, Allison B</au><au>Freishtat, Robert J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs</atitle><jtitle>Journal of translational medicine</jtitle><addtitle>J Transl Med</addtitle><date>2019-07-22</date><risdate>2019</risdate><volume>17</volume><issue>1</issue><spage>232</spage><epage>232</epage><pages>232-232</pages><artnum>232</artnum><issn>1479-5876</issn><eissn>1479-5876</eissn><abstract>Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.
We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m
) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) adipocyte-derived extracellular vesicle microRNAs in serum; (3) the role of visceral adipose tissue extracellular vesicle in regulation of cholesterol efflux and cholesterol efflux gene expression in THP-1 macrophages in vitro.
Efflux capacity was significantly associated with HDL (r = 0.30, p = 0.01) and LDL (r = 0.33, p = 0.005) particle size. Multivariate-analysis identified six microRNAs associated (p < 0.05) with cholesterol efflux capacity: miR-3129-5p (Beta = 0.695), miR-20b (0.430), miR9-5p (0.111), miR-320d (- 0.190), miR301a-5p (0.042), miR-155-5p (0.004). In response to increasing concentrations (1 μg/mL vs. 3 μg/mL) of VAT extracellular vesicle, cholesterol efflux (66% ± 10% vs. 49% ± 2%; p < 0.01) and expression of ABCA1 (FC = 1.9 ± 0.8 vs 0.5 ± 0.2; p < 0.001), CD36 (0.7 ± 0.4 vs. 2.1 ± 0.8, p = 0.02), CYP27A1 (1.4 ± 0.4 vs. 0.9 ± 0.5; p < 0.05), and LXRA (1.8 ± 1.1 vs. 0.5 ± 0.2; p < 0.05) was altered in THP-1 cells in vitro.
Adipocyte-derived extracellular vesicle microRNAs may, in part, be involved macrophage cholesterol efflux regulation.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>31331347</pmid><doi>10.1186/s12967-019-1980-6</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4478-2441</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Adolescent obesity Biochemistry Cardiovascular diseases Care and treatment Cholesterol Cholesterol Efflux Cholesterol metabolism Cytochrome P-450 Extracellular vesicle Gene expression Genes Genetic aspects Health aspects Low density lipoproteins Macrophages Medical research MicroRNA microRNAs Obesity |
| title | Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs |
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