Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride

Non-invasive delivery of hyaluronan into the stratum corneum (SC) is extremely difficult because of its high molecular weight and the strong barrier of the SC. We developed a safe method of administering hyaluronan into the human SC and determined its penetration route. The amount of hyaluronan that...

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Bibliographic Details
Published in:Scientific reports 2023-07, Vol.13 (1), p.10782-10782, Article 10782
Main Authors: Fujii, Mika Y., Okishima, Anna, Ichiwata, Hiroko S., Oka, Takashi
Format: Article
Language:English
Subjects:
631/154/152
631/154/433
Epidermis
Humanities and Social Sciences
Humans
Hyaluronic Acid
Magnesium
Magnesium Chloride
Molecular Weight
multidisciplinary
Science
Science (multidisciplinary)
Skin
Stratum corneum
Topical application
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Summary:Non-invasive delivery of hyaluronan into the stratum corneum (SC) is extremely difficult because of its high molecular weight and the strong barrier of the SC. We developed a safe method of administering hyaluronan into the human SC and determined its penetration route. The amount of hyaluronan that penetrated into the SC was 1.5–3 times higher in the presence of magnesium chloride hexahydrate (MgCl 2 ) than other metal chlorides. The root-mean-square radius of hyaluronan in water decreased with the addition of MgCl 2 . Moreover, MgCl 2 solutions maintained their dissolved state on a plastic plate for a long time, suggesting that size compaction and inhibition of hyaluronan precipitation on the skin enhanced hyaluronan into the SC. Our results also strongly suggest that an intercellular route contributes to the penetration of hyaluronan from the upper to the middle layer of the SC. No disruption to the SC barrier was observed after continuous use once a day for 1 month, demonstrating the potential of our method for the safe, topical application of hyaluronan.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-37718-5