Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats

• Published in: Acta cirúrgica brasileira 2017-03, Vol.32 (3), p.203-210
• Main Authors: Lemos Neto, Sylvio Valença de, Vianna, Isabela Galvão, Castiglia, Yara Marcondes Machado, Golim, Marjorie de Assis, Souza, Aparecida Vitória Gonçalves de, Carvalho, Lídia Raquel de, Deffune, Elenice, Nascimento Junior, Paulo do, Módolo, Norma Sueli Pinheiro, Vianna, Pedro Thadeu Galvão
• Format: Article
• Language: English
• Subjects: Apoptosis; Cyclosporine; Hyperglycemia; Kidney; Rats; Reperfusion Injury
• ISSN: 0102-8650; 0102-8650
• DOI: 10.1590/s0102-865020170030000004

Abstract Purpose: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats. Methods: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells. Results: Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury. Conclusion: In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.