Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mec...

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Published in:Stem cell research & therapy 2020-06, Vol.11 (1), p.240-240, Article 240
Main Authors: Shi, Zheng-Liang, Zhang, Hua, Fan, Zhi-Yong, Ma, Wei, Song, Yong-Zhou, Li, Ming, Li, Tong-Qiu, Cao, Shu-Xing, Feng, Guo-Jun
Format: Article
Language:English
Subjects:
ADSCs
Alkaline phosphatase
Antisense RNA
Calcium
Cell Differentiation
Gene expression
GRM5
Health savings accounts
Humans
LINC00314
MicroRNA
MicroRNAs
MicroRNAs - genetics
miR-129-5p
miRNA
Osteogenesis - genetics
Osteogenic differentiation
Osteoporosis
Phosphatases
Polymerase chain reaction
RNA, Long Noncoding - genetics
Signal transduction
Stem cell research
Stem Cells
Wnt protein
Wnt Signaling Pathway
β-Catenin
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description Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.
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Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-020-01754-z</identifier><identifier>PMID: 32552820</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>ADSCs ; Alkaline phosphatase ; Antisense RNA ; Calcium ; Cell Differentiation ; Gene expression ; GRM5 ; Health savings accounts ; Humans ; LINC00314 ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; miR-129-5p ; miRNA ; Osteogenesis - genetics ; Osteogenic differentiation ; Osteoporosis ; Phosphatases ; Polymerase chain reaction ; RNA, Long Noncoding - genetics ; Signal transduction ; Stem cell research ; Stem Cells ; Wnt protein ; Wnt Signaling Pathway ; β-Catenin</subject><ispartof>Stem cell research &amp; therapy, 2020-06, Vol.11 (1), p.240-240, Article 240</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. 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therapy</jtitle><addtitle>Stem Cell Res Ther</addtitle><date>2020-06-17</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>240</spage><epage>240</epage><pages>240-240</pages><artnum>240</artnum><issn>1757-6512</issn><eissn>1757-6512</eissn><abstract>Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32552820</pmid><doi>10.1186/s13287-020-01754-z</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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subjects ADSCs
Alkaline phosphatase
Antisense RNA
Calcium
Cell Differentiation
Gene expression
GRM5
Health savings accounts
Humans
LINC00314
MicroRNA
MicroRNAs
MicroRNAs - genetics
miR-129-5p
miRNA
Osteogenesis - genetics
Osteogenic differentiation
Osteoporosis
Phosphatases
Polymerase chain reaction
RNA, Long Noncoding - genetics
Signal transduction
Stem cell research
Stem Cells
Wnt protein
Wnt Signaling Pathway
β-Catenin
title Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway
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