Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

• Published in: Journal of functional foods 2020-11, Vol.74, p.104198, Article 104198
• Main Authors: Xiao, Zhenbang, Yang, Shengtao, Chen, Jiali, Li, Chengyong, Zhou, Chunxia, Hong, Pengzhi, Sun, Shengli, Qian, Zhong-Ji
• Format: Article
• Language: English
• Subjects: HaCaT cells; Oxidative stress; Photoaging; Trehalose; UVB
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2020.104198

[Display omitted] •MMP-1, -3 and -9 were reduced by Trehalose.•Anti-photoaging effect via affected the MAPKs, AP-1 and NF-κB pathways.•Enhance procollagen I content via TGF-β/Smad pathways.•Trehalose has a therapeutic effectiveness in prevention of UVB. Ultraviolet radiation B cause photoaging of the skin by enhancing the high expression of matrix metalloproteinases (MMPs) to degrade collagen and inhibit the synthesis of procollagen. Trehalose (TRE), a widely occurring natural disaccharide, has been shown to play a cytoprotective role in many pathological processes. However, few studies have evaluated whether it has anti-photoaging potential. In this study, we investigated the anti-photoaging and mechanism of action of TRE using Human immortalized keratinocytes (HaCaT) cells. The results indicated that TRE pretreatment effectively scavenges reactive oxygen species (ROS) accumulation induced by UVB and increases the content of certain endogenous antioxidant factors in HaCaT cells. TRE suppressed UVB-induced MMP expression by blocking mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1) and NF-κB signaling. TRE treatment increased procollagen I synthesis via activating transforming growth factor-β (TGF-β)/Smad pathway and demonstrated that TRE may be a potential natural disaccharide for photoaging therapy in the future.