Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice

Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectio...

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Bibliographic Details
Published in:Viruses 2019-02, Vol.11 (2), p.161
Main Authors: Logue, James, Vargas Licona, Walter, Cooper, Timothy K, Reeder, Becky, Byrum, Russel, Qin, Jing, Deiuliis Murphy, Nicole, Cong, Yu, Bonilla, Amanda, Sword, Jennifer, Weaver, Wade, Kocher, Gregory, Olinger, Gene G, Jahrling, Peter B, Hensley, Lisa E, Bennett, Richard S
Format: Article
Language:English
Subjects:
Animal models
Animals
Animals, Suckling
Antibiotics
Breast milk
Breastfeeding & lactation
Cell culture
Cell Line
Cell Line, Tumor
Chlorocebus aethiops
Disease transmission
Ebola virus
Ebolavirus
Ebolavirus - isolation & purification
Hemorrhagic Fever, Ebola - virology
Huh7
Humans
Infectivity
Laboratory animals
Liver cancer
Macrophages
Macrophages - virology
Medical screening
Mice
Mice, Inbred BALB C
Milk, Human - virology
Monocyte Derived Macrophages
Monocytes
Public health
Semen
Semen - virology
Sensitivity and Specificity
Suckling behavior
Toxicity
Vero
Vero Cells
Viral infections
Virology - methods
Virus isolation
Viruses
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Summary:Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation.
ISSN:1999-4915
1999-4915
DOI:10.3390/v11020161