Evidence of Nrf2/Keap1 Signaling Regulation by Mitochodria-Generated Reactive Oxygen Species in RGK1 Cells

It has been known that reactive oxygen species (ROS) are generated from the mitochondrial electron transport chain (ETC). Majima et al. proved that mitochondrial ROS (mtROS) caused apoptosis for the first time in 1998 (Majima et al. J Biol Chem, 1998). It is speculated that mtROS can move out of the...

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Published in:Biomolecules (Basel, Switzerland) Switzerland), 2023-02, Vol.13 (3), p.445
Main Authors: Indo, Hiroko P, Masuda, Daisuke, Sriburee, Sompong, Ito, Hiromu, Nakanishi, Ikuo, Matsumoto, Ken-Ichiro, Mankhetkorn, Samlee, Chatatikun, Moragot, Surinkaew, Sirirat, Udomwech, Lunla, Kawakami, Fumitaka, Ichikawa, Takafumi, Matsui, Hirofumi, Tangpong, Jitbanjong, Majima, Hideyuki J
Format: Article
Language:English
Subjects:
Alzheimer's disease
Analysis
Apoptosis
Cancer therapies
Cell lines
cell signaling
Cells
Electron transport
Electron transport chain
gastric mucosal cells
GATA-1 protein
GATA-3 protein
Genes
Humans
Identification and classification
Immunocytochemistry
Ischemia
Kelch-Like ECH-Associated Protein 1 - genetics
Kelch-Like ECH-Associated Protein 1 - metabolism
Manganese
Mitochondria
Mitochondrial DNA
NF-E2-Related Factor 2 - genetics
NF-E2-Related Factor 2 - metabolism
Oxidative stress
Radiation
Reactive oxygen species
Reactive Oxygen Species - metabolism
RNA
Signal Transduction
Superoxide dismutase
Superoxide Dismutase - genetics
Superoxide Dismutase - metabolism
Transfection
tumorized cells
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Summary:It has been known that reactive oxygen species (ROS) are generated from the mitochondrial electron transport chain (ETC). Majima et al. proved that mitochondrial ROS (mtROS) caused apoptosis for the first time in 1998 (Majima et al. J Biol Chem, 1998). It is speculated that mtROS can move out of the mitochondria and initiate cellular signals in the nucleus. This paper aims to prove this phenomenon by assessing the change in the amount of manganese superoxide dismutase (MnSOD) by MnSOD transfection. Two cell lines of the same genetic background, of which generation of mtROS are different, i.e., the mtROS are more produced in RGK1, than in that of RGM1, were compared to analyze the cellular signals. The results of immunocytochemistry staining showed increase of Nrf2, Keap1, HO-1 and 2, MnSOD, GCL, GST, NQO1, GATA1, GATA3, GATA4, and GATA5 in RGK1 compared to those in RGM1. Transfection of human MnSOD in RGK1 cells showed a decrease of those signal proteins, suggesting mtROS play a role in cellular signals in nucleus.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom13030445