Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene

Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family,...

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Bibliographic Details
Published in:Czech Journal of Genetics and Plant Breeding 2016-01, Vol.52 (2), p.77-82
Main Authors: XUE, Gang, LU, Li-Ming, YANG, Tie-Zhao, LI, Xiao-Hui, XING, Xue-Xia, XU, Shi-Xiao
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
atcipk23
Calcineurin
Cbl protein
Cellular stress response
Cultivars
Genetic transformation
Hydroponics
k+ uptake
Kinases
low-k+ tolerance
Nicotiana tabacum
Potassium
Protein kinase
Protein kinase C
Proteins
Tobacco
Transgenic plants
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Summary:Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress.
ISSN:1212-1975
1805-9325
DOI:10.17221/155/2015-CJGPB