Innovative targets of the lncRNA-miR-mRNA network in response to low-dose aspirin in breast cancer patients

• Published in: Scientific reports 2022-07, Vol.12 (1), p.12054-12054, Article 12054
• Main Authors: Alipour, Sadaf, Khalighfard, Solmaz, Khori, Vahid, Amiriani, Taghi, Tajaldini, Mahboubeh, Dehghan, Mohammad, Sadani, Somayeh, Omranipour, Ramesh, Vahabzadeh, Gelareh, Eslami, Bita, Alizadeh, Ali Mohammad
• Format: Article
• Language: English
• Subjects: 631/114; 631/136; 631/1647; 631/208; 631/250; 631/337; 631/67; 631/80; 631/92; 692/4017; 692/4028; 692/53; 692/699; Aspirin; Breast cancer; Chemotherapy; Humanities and Social Sciences; Interleukin 17; mRNA; multidisciplinary; Next-generation sequencing; Non-coding RNA; Patients; Science; Science (multidisciplinary); Survival; Tumor suppressor genes; Tumors; γ-Interferon
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-16398-7

This study aimed to investigate innovative targets in breast cancer patients by considering the interaction of the lncRNA-miR-mRNA network in response to low-dose aspirin. The candidate miRs were first taken from the GEO and TCGA databases. Then, the candidate network was constructed using the high-throughput sequencing data. The expression levels of candidate targets were finally measured using Real-Time PCR in luminal A breast cancer patients undergoing aspirin (80 mg daily for three months) and non-aspirin groups during chemotherapy after surgery. The expression levels of TGFβ, IL-17, IFNγ, and IL-β proteins were measured using the ELISA technique. 5 lncRNAs, 12 miRs, and 10 genes were obtained in the bioinformatic phase. A significant expression increase of the candidate tumor suppressor lncRNAs, miRs, and genes and a substantial expression decrease of the candidate onco-lncRNAs, oncomiRs, and oncogenes were achieved after the aspirin consumption. Unlike the non-aspirin group, the expression levels of TGFβ, IL-17, IFNγ, and IL-β proteins were significantly decreased following aspirin consumption. The Kaplan–Meier analysis indicated a longer overall survival rate in the patients after aspirin consumption. Our results showed that the lncRNA-miR-mRNA network might be a significant target for aspirin; their expression changes may be a new strategy with potential efficacy for cancer therapy or prevention.