Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

The infectious pathogen Staphylococcus aureus (S. aureus) is primarily associated with osteomyelitis. Hydrogen peroxide drainage is an effective antimicrobial treatment that has been adopted to combat S. aureus infections. Previous investigations have indicated that the antisense RNA (asRNA) strateg...

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Bibliographic Details
Published in:BMC microbiology 2021-05, Vol.21 (1), p.1-160, Article 160
Main Authors: Wu, Shizhou, Liu, Yunjie, Lei, Lei, Zhang, Hui
Format: Article
Language:English
Subjects:
Animal models
Antiinfectives and antibacterials
Antimicrobial agents
Antisense
Antisense RNA
Bacteria
Biofilms
Biomedical materials
Care and treatment
Chemical properties
Extracellular matrix
Gene expression
Genes
Genetic aspects
Genetic transcription
Hydrogen
Hydrogen peroxide
Kinases
Metabolism
Microorganisms
Osteomyelitis
Physiological aspects
Protein expression
Proteins
Ribonucleic acid
RNA
sarA
Signal transduction
Staphylococcus aureus
Staphylococcus aureus infections
Transcription
Virulence
YycFG
Zinc oxides
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Summary:The infectious pathogen Staphylococcus aureus (S. aureus) is primarily associated with osteomyelitis. Hydrogen peroxide drainage is an effective antimicrobial treatment that has been adopted to combat S. aureus infections. Previous investigations have indicated that the antisense RNA (asRNA) strategy negatively modulates S. aureus YycFG TCS, and it significantly disrupts biofilm formation. However, the effects of the antisense yycG RNA (ASyycG) strategy on the susceptibility of biofilm-producing S. aureus to hydrogen peroxide and the mechanisms underlying this effect have not been elucidated to date. Overexpression of ASyycG inhibited the transcription of biofilm formation-related genes, including sarA and icaA. Additionally, the CFU counts and the live bacterial ratios of ASyycG biofilm-producing S. aureus treated with H.sub.2O.sub.2 were notably reduced across the groups. Notably, the predicted promoter regions of the sarA and icaA genes were directly regulated by YycF. ASyycG was observed to sensitize biofilm-producing S. aureus to H.sub.2O.sub.2 intervention synergistically via the sarA and thus may represent a supplementary strategy for managing osteomyelitis. However, future in-depth studies should attempt to replicate our findings in animal models, such as the rat osteomyelitis model.
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-021-02218-x