Special Issue on ‘Hide and Seek of Soil Microbes—Who Is Where with Whom and Why?’

[...]micro-landscape structures could support the persistence of less competitive species in the ecosystem. The author pinpoints the fact that the still unexplored role of microbial diversity—using the energy from the soil organic substrates, and, therefore, the who, where, with whom, and why of man...

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Bibliographic Details
Published in:Applied sciences 2022-08, Vol.12 (15), p.7693
Main Authors: Probst, Maraike, Ascher-Jenull, Judith
Format: Article
Language:English
Subjects:
Aquatic ecosystems
Aquatic environment
Bioinformatics
Case studies
Deoxyribonucleic acid
Design of experiments
DNA
Ecosystems
Energy
Environmental DNA
Experimental design
Metabolism
Microbiota
Microorganisms
n/a
Organic soils
Soil analysis
Soil microorganisms
Soil sciences
Substrates
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Summary:[...]micro-landscape structures could support the persistence of less competitive species in the ecosystem. The author pinpoints the fact that the still unexplored role of microbial diversity—using the energy from the soil organic substrates, and, therefore, the who, where, with whom, and why of managing that energy—could be assessed by unraveling the nature of the soil organic substrates and by monitoring the energy released by the soil microbial metabolism (decomposition vs. assimilation of soil organic substrates). [...]the author is right that soil organic content/matter needs differentiation in order to explain the soil carbon cycle in a more appropriate, meaningful and detailed manner. 3. In this regard, e.g., the discriminatory study of the extracellular (exDNA) and intracellular (iDNA) fractions of the total environmental DNA pool (eDNA), might be a promising approach to (i) further increase the overall information stored about microbiota in soil, e.g., [6], and other environments [7], including specific habitats such as deadwood [8], and (ii) to correctly interpret, critically discuss, and draw relevant conclusions of DNA-based results. [...]in the era of culture-independent high-throughput molecular analyses coupled with advanced ecological networking via bioinformatics, the basic, defining steps of any experiment must be taken seriously and correctly, i.e., the experimental design including sampling strategy, soil storage and DNA extraction methods (reviewed by [9,10]). [...]the combination of culture-dependent and culture-independent analyses of soil microbiota, and that of all other ecosystems, including aquatic systems, is recommended now more than ever, and can be considered key to the in-depth answer to the question:
ISSN:2076-3417
2076-3417
DOI:10.3390/app12157693