The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet

DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verifie...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-01, Vol.25 (2), p.1118
Main Authors: Weng, Jiamin, Wang, Hui, Cheng, Dayou, Liu, Tianjiao, Zeng, Deyong, Dai, Cuihong, Luo, Chengfei
Format: Article
Language:English
Subjects:
Abortion
Analysis
Beta vulgaris - genetics
Carbohydrate metabolism
cytoplasmic male sterility
DNA
DNA Methylation
DNA sequencing
Epigenetic inheritance
Epigenetics
Gene expression
Genomes
Genomics
Metabolism
methylated gene
Methylation
Mitochondrial DNA
Nucleotide sequencing
Plant Infertility - genetics
Polypeptides
sugar beet
Sugars
Sulfites
Vegetables
whole-genome bisulfite sequencing
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Summary:DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verified by bisulfite transformation, indicating that the results of WGBS technology were reliable. In addition, 12 differential methylation genes (DMGs) were identified, which were related to carbohydrate and energy metabolism, pollen wall development, and endogenous hormone regulation. Quantitative real-time PCR (qRT-PCR) showed that 75% of DMG expression levels showed negative feedback with methylation level, indicating that DNA methylation can affect gene expression to a certain extent. In addition, we found hypermethylation inhibited gene expression, which laid a foundation for further study on the molecular mechanism of DNA methylation at the epigenetic level in sugar beet male sterility.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25021118