Chromium Induces Toxicity at Different Phenotypic, Physiological, Biochemical, and Ultrastructural Levels in Sweet Potato (Ipomoea batatas L.) Plants

• Published in: International journal of molecular sciences 2022-11, Vol.23 (21), p.13496
• Main Authors: Kumar, Sunjeet, Wang, Mengzhao, Fahad, Shah, Qayyum, Abdul, Chen, Yanli, Zhu, Guopeng
• Format: Article
• Language: English
• Subjects: Accumulation; antioxidant defense system; Antioxidants; Biomass; Carotenoids; Chlorophyll; Chromium; Enzymatic activity; Heavy metals; Hydrogen peroxide; Ipomoea batatas; Lipid peroxidation; Lipids; Low level; oxidative damage; Photosynthesis; photosynthetic pigments; Physiology; Plant growth; Potatoes; Rape plants; Reactive oxygen species; Size reduction; Stomata; stomatal traits; Superoxide dismutase; sweet potato; Sweet potatoes; Toxicity; Ultrastructure
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232113496

Crop productivity is enormously exposed to different environmental stresses, among which chromium (Cr) stress raises considerable concerns and causes a serious threat to plant growth. This study explored the toxic effect of Cr on sweet potato plants. Plants were hydroponically grown, and treatments of 0, 25, 50, 100, and 200 µM Cr were applied for seven days. This study exhibited that a low level of Cr treatment (25 µM) enhanced the growth, biomass, photosynthesis, osmolytes, antioxidants, and enzyme activities. However, significant deleterious effects in growth, biomass, photosynthetic attributes, antioxidants, and enzymes were observed at higher levels of Cr treatment. The remarkable reduction in plant growth traits was associated with the over-accumulation of H2O2 and MDA contents (410% and 577%, respectively) under the highest rate of Cr (200 µM). Under 200 µM Cr, the uptake in the roots were 27.4 mg kg−1 DW, while in shoots were 11 mg kg−1 DW with the highest translocation rate from root to shoot was 0.40. The results showed that the higher accumulation of Cr negatively correlated with the phenotypic and physiological parameters. It may be proposed that Cr toxicity causes oxidative damage as sustained by augmented lipid peroxidation, reactive oxygen species, and reduced photosynthetic rate, chlorophyll, and stomatal traits. The chloroplastic ultrastructure was damaged, and more apparent damage and size reduction were observed at higher Cr levels. Furthermore, aggregated Cr concentration positively correlates with the increase of osmolytes and superoxide dismutase (SOD) activity in the leaves of sweet potato. Moreover, improved osmolytes and SOD do not help protect sweet potato against high Cr stress. Overall, these findings will improve the understanding of the defense mechanisms of sweet potato to Cr stress.