Lifestyles Shape the Cytochrome P450 Repertoire of the Bacterial Phylum Proteobacteria

• Published in: International journal of molecular sciences 2022-05, Vol.23 (10), p.5821
• Main Authors: Msweli, Siphesihle, Chonco, Andiswa, Msweli, Lihle, Syed, Puleng Rosinah, Karpoormath, Rajshekhar, Chen, Wanping, Gront, Dominik, Nkosi, Bridget Valeria Zinhle, Nelson, David R, Syed, Khajamohiddin
• Format: Article
• Language: English
• Subjects: Adaptation; Alphaproteobacteria; Bacteria; Betaproteobacteria; Comparative analysis; Cytochrome; Cytochrome P450; Cytochromes P450; Deltaproteobacteria; Epsilonproteobacteria; Gammaproteobacteria; Gene clusters; genome-data mining; Genomes; Heme; Lifestyles; Metabolism; Metabolites; Nitrogen; Organisms; Oxidation; Pathogens; Phylogenetics; Proteobacteria; Ulcers
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23105821

For the last six decades, cytochrome P450 monooxygenases (CYPs/P450s), heme thiolate proteins, have been under the spotlight due to their regio- and stereo-selective oxidation activities, which has led to the exploration of their applications in almost all known areas of biology. The availability of many genome sequences allows us to understand the evolution of P450s in different organisms, especially in the Bacteria domain. The phenomenon that "P450s play a key role in organisms' adaptation vis a vis lifestyle of organisms impacts P450 content in their genome" was proposed based on studies on a handful of individual bacterial groups. To have conclusive evidence, one must analyze P450s and their role in secondary metabolism in species with diverse lifestyles but that belong to the same category. We selected species of the phylum classes, , , , , and , to address this research gap due to their diverse lifestyle and ancient nature. The study identified that the lifestyle of alpha-, beta-, gamma-, delta-, and epsilon-proteobacterial species profoundly affected P450 profiles in their genomes. The study determined that irrespective of the species associated with different proteobacterial classes, pathogenic species or species adapted to a simple lifestyle lost or had few P450s in their genomes. On the contrary, species with saprophytic or complex lifestyles had many P450s and secondary metabolite biosynthetic gene clusters. The study findings prove that the phenomenon mentioned above is factual, and there is no link between the number and diversity of P450s and the age of the bacteria.