Regulation of subcellular dendritic synapse specificity by axon guidance cues

Neural circuit assembly occurs with subcellular precision, yet the mechanisms underlying this precision remain largely unknown. Subcellular synaptic specificity could be achieved by molecularly distinct subcellular domains that locally regulate synapse formation, or by axon guidance cues restricting...

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Bibliographic Details
Published in:eLife 2019-04, Vol.8
Main Authors: Sales, Emily C, Heckman, Emily L, Warren, Timothy L, Doe, Chris Q
Format: Article
Language:English
Subjects:
Animals
Axon Guidance
Axons
Cues
dendrite
Developmental Biology
Drosophila
Insects
Lasers
Mammals
Microscopy
Models, Neurological
Nerve Net - growth & development
Neural circuitry
Neurons
Neuroscience
Physiological aspects
Sensory neurons
synapse formation
Synapses
Synapses - physiology
Synaptogenesis
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Summary:Neural circuit assembly occurs with subcellular precision, yet the mechanisms underlying this precision remain largely unknown. Subcellular synaptic specificity could be achieved by molecularly distinct subcellular domains that locally regulate synapse formation, or by axon guidance cues restricting access to one of several acceptable targets. We address these models using two neurons: the dbd sensory neuron and the A08a interneuron. In wild-type larvae, dbd synapses with the A08a medial dendrite but not the A08a lateral dendrite. dbd-specific overexpression of the guidance receptors Unc-5 or Robo-2 results in lateralization of the dbd axon, which forms anatomical and functional monosynaptic connections with the A08a lateral dendrite. We conclude that axon guidance cues, not molecularly distinct dendritic arbors, are a major determinant of dbd-A08a subcellular synapse specificity.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.43478