Circulating microRNAs and endothelial cell migration rate are associated with metabolic syndrome and fitness level in postmenopausal African American women

Postmenopausal African American women are at elevated risk for metabolic syndrome (MetS), which predisposes them to cardiovascular disease and other chronic diseases. Circulating microRNAs (ci‐miR) are potential mediators of cardiometabolic diseases also impacted by cardiorespiratory fitness (CRF) l...

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Bibliographic Details
Published in:Physiological reports 2019-07, Vol.7 (14), p.e14173-n/a
Main Authors: Sapp, Ryan M., Shill, Daniel D., Dash, Chiranjeev, Hicks, Jennifer C., Adams‐Campbell, Lucile L., Hagberg, James M.
Format: Article
Language:English
Subjects:
Adult
African Americans
Black or African American
Body weight
Cardiorespiratory Fitness
Cardiovascular disease
Cardiovascular diseases
Cell adhesion & migration
Cell migration
Cell Movement
circulating microRNA
Circulating MicroRNA - blood
Endothelial cells
Endothelial Cells - metabolism
Endothelium
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Exercise
Experiments
Female
Gene expression
Heart rate
Humans
menopause
Metabolic syndrome
Metabolic Syndrome - blood
Metabolic Syndrome - epidemiology
MicroRNAs
Middle Aged
miRNA
Obesity
Original Research
Overweight
Physical fitness
Physiology
Post-menopause
Postmenopause - blood
Postmenopause - ethnology
Postmenopause - physiology
Studies
Womens health
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Summary:Postmenopausal African American women are at elevated risk for metabolic syndrome (MetS), which predisposes them to cardiovascular disease and other chronic diseases. Circulating microRNAs (ci‐miR) are potential mediators of cardiometabolic diseases also impacted by cardiorespiratory fitness (CRF) level. Using real‐time quantitative PCR, we compared the expression of vascular‐related ci‐miRs (miR‐21‐5p, miR‐92a‐3p, miR‐126‐5p, miR‐146a‐5p, miR‐150‐5p, miR‐221‐3p) in sedentary, overweight/obese, postmenopausal African American women based on 1) presence (n = 31) or absence (n = 42) of MetS and 2) CRF level (VO2peak) (Very Low 22.0 mL·kg−1·min−1 [n = 18]). Endothelial migration rate in response to subjects’ serum was assessed to determine the effect of circulating blood‐borne factors on endothelial repair. Ci‐miR‐21‐5p was the only ci‐miR that differed between women with MetS compared to those without MetS (0.93 ± 0.43 vs. 1.28 ± 0.71, P = 0.03). There were borderline significant differences (P = 0.06–0.09) in ci‐miR‐21‐5p, 126‐5p, and 221‐3p levels between the CRF groups, and these three ci‐miRs correlated with VO2peak (r = −0.25 to −0.28, P 
ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.14173